LOCUS       BBa_S04065              1376 bp    DNA     linear       27-May-2024
DEFINITION  K137049:S03998
ACCESSION   BBa_S04065
VERSION     BBa_S04065.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=tetR promotoer
     promoter        1..450
                     /label=BBa_K137049
     misc_feature    459..475
                     /label=IRR out
     misc_feature    477..493
                     /label=IRR in
     misc_feature    459..493
                     /label=BBa_K137008
     misc_feature    506..509
                     /label=conserved
     RBS             502..513
                     /label=BBa_B0034
     CDS             520..1239
                     /label=GFP protein
     CDS             520..1239
                     /label=BBa_E0040
     stem_loop       1259..1302
                     /label=stem_loop
     misc_feature    1248..1327
                     /label=BBa_B0010
     terminator      1248..1327
                     /label=BBa_B0010
     stem_loop       1343..1362
                     /label=T7 TE
     misc_feature    1369..1369
                     /label=stop
     polyA_site      1363..1376
                     /label=polya
     misc_feature    1336..1376
                     /label=BBa_B0012
     terminator      1336..1376
                     /label=BBa_B0012
ORIGIN
        1 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactctag
       61 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      181 ttgatgcctg gctctagtag tgatctacac tagcactatc agtgttatta agctactaaa
      241 gcgtagtttt cgtcgtttgc agcggaccca ctttcacatt taagttgttt ttctaatccg
      301 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat
      361 tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct
      421 ttagcgactt gatgctcttg atcttccaat tactagagaa gatgaaacat ttggggccaa
      481 actgtccata ttatactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt
      541 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      601 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      661 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      721 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      781 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      841 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      901 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      961 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     1021 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1081 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1141 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     1201 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     1261 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1321 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//