LOCUS       BBa_S04066              1576 bp    DNA     linear       26-May-2024
DEFINITION  K137050:S03998
ACCESSION   BBa_S04066
VERSION     BBa_S04066.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=tetR promoter
     promoter        1..650
                     /label=BBa_K137050
     misc_feature    659..675
                     /label=IRR out
     misc_feature    677..693
                     /label=IRR in
     misc_feature    659..693
                     /label=BBa_K137008
     misc_feature    706..709
                     /label=conserved
     RBS             702..713
                     /label=BBa_B0034
     CDS             720..1439
                     /label=GFP protein
     CDS             720..1439
                     /label=BBa_E0040
     stem_loop       1459..1502
                     /label=stem_loop
     misc_feature    1448..1527
                     /label=BBa_B0010
     terminator      1448..1527
                     /label=BBa_B0010
     stem_loop       1543..1562
                     /label=T7 TE
     misc_feature    1569..1569
                     /label=stop
     polyA_site      1563..1576
                     /label=polya
     misc_feature    1536..1576
                     /label=BBa_B0012
     terminator      1536..1576
                     /label=BBa_B0012
ORIGIN
        1 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactctag
       61 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      181 ttgatgcctg gctctagtag tgatctacac tagcactatc agtgttatta agctactaaa
      241 gcgtagtttt cgtcgtttgc agcggaccca ctttcacatt taagttgttt ttctaatccg
      301 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat
      361 tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct
      421 ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg
      481 ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga
      541 ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca
      601 tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa tactagagaa
      661 gatgaaacat ttggggccaa actgtccata ttatactaga gaaagaggag aaatactaga
      721 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      781 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      841 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      901 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      961 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1021 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1081 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1141 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1201 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1261 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1321 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1381 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1441 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1501 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1561 cctttctgcg tttata
//