LOCUS       BBa_S04067              1776 bp    DNA     linear       19-May-2024
DEFINITION  K137051:S03998
ACCESSION   BBa_S04067
VERSION     BBa_S04067.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=tetR promoter
     promoter        1..850
                     /label=BBa_K137051
     misc_feature    859..875
                     /label=IRR out
     misc_feature    877..893
                     /label=IRR in
     misc_feature    859..893
                     /label=BBa_K137008
     misc_feature    906..909
                     /label=conserved
     RBS             902..913
                     /label=BBa_B0034
     CDS             920..1639
                     /label=GFP protein
     CDS             920..1639
                     /label=BBa_E0040
     stem_loop       1659..1702
                     /label=stem_loop
     misc_feature    1648..1727
                     /label=BBa_B0010
     terminator      1648..1727
                     /label=BBa_B0010
     stem_loop       1743..1762
                     /label=T7 TE
     misc_feature    1769..1769
                     /label=stop
     polyA_site      1763..1776
                     /label=polya
     misc_feature    1736..1776
                     /label=BBa_B0012
     terminator      1736..1776
                     /label=BBa_B0012
ORIGIN
        1 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactctag
       61 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      181 ttgatgcctg gctctagtag tgatctacac tagcactatc agtgttatta agctactaaa
      241 gcgtagtttt cgtcgtttgc agcggaccca ctttcacatt taagttgttt ttctaatccg
      301 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat
      361 tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct
      421 ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg
      481 ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga
      541 ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca
      601 tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc
      661 cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct
      721 aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca
      781 cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc
      841 tctaatgcgc tactagagaa gatgaaacat ttggggccaa actgtccata ttatactaga
      901 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      961 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1021 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1081 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1141 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1201 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1261 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1321 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1381 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1441 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1501 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1561 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1621 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1681 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1741 ctggctcacc ttcgggtggg cctttctgcg tttata
//