LOCUS       BBa_S04089              1840 bp    DNA     linear       21-May-2024
DEFINITION  Q04400:P0451
ACCESSION   BBa_S04089
VERSION     BBa_S04089.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    1..902
                     /label=BBa_Q04400
     misc_feature    915..918
                     /label=conserved
     RBS             911..922
                     /label=BBa_B0034
     CDS             932..1639
                     /label=cI lambda
     misc_feature    1640..1672
                     /label=LVA
     misc_feature    1679..1703
                     /label=Help:Barcodes
     CDS             929..1678
                     /label=BBa_C0051
     stem_loop       1723..1766
                     /label=stem_loop
     misc_feature    1712..1791
                     /label=BBa_B0010
     terminator      1712..1791
                     /label=BBa_B0010
     stem_loop       1807..1826
                     /label=T7 TE
     misc_feature    1833..1833
                     /label=stop
     polyA_site      1827..1840
                     /label=polya
     misc_feature    1800..1840
                     /label=BBa_B0012
     terminator      1800..1840
                     /label=BBa_B0012
     misc_feature    911..1840
                     /label=BBa_P0451
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga
      961 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg
     1021 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt
     1081 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa
     1141 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc
     1201 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca
     1261 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt
     1321 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat
     1381 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc
     1441 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac
     1501 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta
     1561 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg
     1621 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg
     1681 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
     1741 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1801 cacactggct caccttcggg tgggcctttc tgcgtttata
//