LOCUS       BBa_S04092              1992 bp    DNA     linear       14-Jun-2024
DEFINITION  S03975:Q04400
ACCESSION   BBa_S04092
VERSION     BBa_S04092.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1075..1078
                     /label=conserved
     RBS             1071..1082
                     /label=BBa_B0034
     misc_feature    1095..1098
                     /label=conserved
     RBS             1091..1102
                     /label=BBa_B0034
     CDS             1112..1728
                     /label=tetR
     misc_feature    1729..1762
                     /label=SsrA
     misc_feature    1769..1793
                     /label=Help:Barcodes
     CDS             1109..1768
                     /label=BBa_C0040
     stem_loop       1813..1856
                     /label=stem_loop
     misc_feature    1802..1881
                     /label=BBa_B0010
     terminator      1802..1881
                     /label=BBa_B0010
     stem_loop       1897..1916
                     /label=T7 TE
     misc_feature    1923..1923
                     /label=stop
     polyA_site      1917..1930
                     /label=polya
     misc_feature    1890..1930
                     /label=BBa_B0012
     terminator      1890..1930
                     /label=BBa_B0012
     misc_feature    1939..1957
                     /label=TetR 1
     misc_feature    1939..1992
                     /label=BBa_R0040
     promoter        1958..1963
                     /label=-35
     misc_feature    1964..1982
                     /label=TetR 2
     promoter        1981..1986
                     /label=-10
     promoter        1939..1992
                     /label=BBa_R0040
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     1081 aatactagag aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa
     1141 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     1201 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     1261 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     1321 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     1381 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     1441 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
     1501 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
     1561 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
     1621 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
     1681 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga
     1741 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga
     1801 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1861 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1921 tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat cagtgataga
     1981 gatactgagc ac
//