LOCUS       BBa_S04138              1770 bp    DNA     linear       17-May-2024
DEFINITION  cI QPI with GFP reporter system
ACCESSION   BBa_S04138
VERSION     BBa_S04138.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             13..726
                     /label=cIts
     CDS             13..726
                     /label=BBa_K098997
     misc_feature    1..726
                     /label=BBa_K098998
     stem_loop       742..761
                     /label=T7 TE
     misc_feature    768..768
                     /label=stop
     polyA_site      762..775
                     /label=polya
     misc_feature    735..775
                     /label=BBa_B0012
     terminator      735..775
                     /label=BBa_B0012
     stem_loop       796..818
                     /label=stem_loop
     misc_feature    784..829
                     /label=BBa_B0011
     terminator      784..829
                     /label=BBa_B0011
     terminator      735..829
                     /label=BBa_B0014
     promoter        875..880
                     /label=-10
     promoter        852..857
                     /label=-35
     misc_feature    862..878
                     /label=OR1
     misc_feature    838..854
                     /label=OR2
     misc_feature    838..886
                     /label=BBa_R0051
     promoter        838..886
                     /label=BBa_R0051
     RBS             895..907
                     /label=RBS-3\Weak
     RBS             901..904
                     /label=RBS
     misc_feature    895..907
                     /label=BBa_B0032
     RBS             895..907
                     /label=BBa_B0032
     CDS             914..1633
                     /label=GFP protein
     CDS             914..1633
                     /label=BBa_E0040
     stem_loop       1653..1696
                     /label=stem_loop
     misc_feature    1642..1721
                     /label=BBa_B0010
     terminator      1642..1721
                     /label=BBa_B0010
     stem_loop       1737..1756
                     /label=T7 TE
     misc_feature    1763..1763
                     /label=stop
     polyA_site      1757..1770
                     /label=polya
     misc_feature    1730..1770
                     /label=BBa_B0012
     terminator      1730..1770
                     /label=BBa_B0012
     misc_feature    838..1770
                     /label=BBa_K098991
ORIGIN
        1 aaagaggaga aaatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
       61 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      121 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      181 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      241 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
      301 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      361 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
      421 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
      481 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
      541 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
      601 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
      661 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt
      721 ggctgatact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
      781 gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttt actagagtaa
      841 caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact agagtcacac
      901 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      961 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1021 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1081 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1141 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1201 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1261 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1321 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1381 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1441 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1501 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1561 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1621 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1681 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1741 caccttcggg tgggcctttc tgcgtttata
//