LOCUS BBa_S04186 3736 bp DNA linear 14-May-2024 DEFINITION I715038:I2035 ACCESSION BBa_S04186 VERSION BBa_S04186.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 promoter 2724..2751 /label=putative E. coli promoter CDS 227..2881 /label=BBa_I2032 promoter 2890..2909 /label=BBa_R0183 fragment misc_feature 2909..2933 /label=lacI binding site misc_feature 2920..2920 /label=Center of binding site symmetry promoter 2890..2933 /label=BBa_R0184 RBS 2942..2954 /label=RBS-3\Weak RBS 2948..2951 /label=RBS misc_feature 2942..2954 /label=BBa_B0032 RBS 2942..2954 /label=BBa_B0032 CDS 2961..3680 /label=GFP protein CDS 2961..3680 /label=BBa_E0040 terminator 3689..3736 /label=BBa_B0016 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acacgattaa 241 catcgctaag aacgacttct ctgacatcga actggctgct atcccgttca acactctggc 301 tgaccattac ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga 361 gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc 421 ggataacgct gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat 481 caacgactgg tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt 541 cctgcaagaa atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg 601 cctaaccagt gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat 661 tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa 721 cgttgaggaa caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt 781 tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca 841 taaggaagac tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg 901 aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga 961 actcgcacct gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc 1021 tccgatgttc caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg 1081 ctattgggct aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact 1141 gatgcgctac gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa 1201 caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa 1261 gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga 1321 agacatcgac atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta 1381 ccgcaaggac aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc 1441 caataagttt gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg 1501 tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac 1561 gctggcgaaa ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc 1621 aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa 1681 ccacgagaac atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca 1741 agattctccg ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg 1801 cctgagctat aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca 1861 cttctccgcg atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga 1921 aaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga 1981 cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat 2041 ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt 2101 tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg 2161 cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct 2221 gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt 2281 gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct 2341 gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt 2401 gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac 2461 gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa 2521 agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag 2581 ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga 2641 atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt 2701 caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt 2761 ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc 2821 ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaata 2881 atactagagt catacgactc actatagggg aattgtgagc ggataacaat tcctactaga 2941 gtcacacagg aaagtactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 3001 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 3061 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 3121 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 3181 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 3241 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 3301 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 3361 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 3421 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 3481 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 3541 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 3601 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 3661 atgaactata caaataataa tactagagct agcataaccc cttggggcct ctaaacgggt 3721 cttgaggggt tttttg //