LOCUS       BBa_S04191              1533 bp    DNA     linear       17-May-2024
DEFINITION  I7101:I6413
ACCESSION   BBa_S04191
VERSION     BBa_S04191.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..801
                     /label=GFP protein
     CDS             82..801
                     /label=BBa_E0040
     stem_loop       821..864
                     /label=stem_loop
     misc_feature    810..889
                     /label=BBa_B0010
     terminator      810..889
                     /label=BBa_B0010
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
     misc_feature    1..938
                     /label=BBa_I7101
     promoter        956..961
                     /label=-35
     promoter        979..984
                     /label=-10
     misc_feature    966..979
                     /label=Zif23
     misc_feature    947..955
                     /label=consensus
     misc_feature    991..991
                     /label=(+1)
     promoter        947..991
                     /label=BBa_R2000
     misc_feature    1004..1007
                     /label=conserved
     RBS             1000..1011
                     /label=BBa_B0034
     misc_feature    1018..1032
                     /label=lacZ gene fragment
     CDS             1018..1371
                     /label=lacZ alpha
     promoter        1043..1052
                     /label=T3 promoter
     promoter        1190..1208
                     /label=T7 promoter
     misc_feature    1095..1095
                     /label=C
     misc_feature    1098..1098
                     /label=T
     misc_feature    1104..1104
                     /label=A
     misc_feature    1125..1125
                     /label=C
     misc_feature    1128..1128
                     /label=G
     misc_feature    1216..1365
                     /label=lacZ gene fragment
     misc_feature    1365..1365
                     /label=inserted T
     misc_feature    1372..1396
                     /label=barcode
     misc_feature    1018..1396
                     /label=BBa_E0035
     stem_loop       1416..1459
                     /label=stem_loop
     misc_feature    1405..1484
                     /label=BBa_B0010
     terminator      1405..1484
                     /label=BBa_B0010
     stem_loop       1500..1519
                     /label=T7 TE
     misc_feature    1526..1526
                     /label=stop
     polyA_site      1520..1533
                     /label=polya
     misc_feature    1493..1533
                     /label=BBa_B0012
     terminator      1493..1533
                     /label=BBa_B0012
     terminator      1405..1533
                     /label=BBa_B0015
     misc_feature    1000..1533
                     /label=BBa_E0435
     misc_feature    947..1533
                     /label=BBa_I6413
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagtt tattcttgac
      961 atggtcccac gcgcgtggga tactacgtca gtactagaga aagaggagaa atactagatg
     1021 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc
     1081 accgcggtgg cggcagcact agagctagtg gatcccccgg gctgtagaaa ttcgatatca
     1141 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     1201 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1261 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1321 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaattaata acgctgatag
     1381 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1441 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1501 gctcaccttc gggtgggcct ttctgcgttt ata
//