LOCUS BBa_S04192 4884 bp DNA linear 14-May-2024 DEFINITION I20279:I2035 ACCESSION BBa_S04192 VERSION BBa_S04192.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 promoter 3735..3762 /label=putative E. coli promoter CDS 1238..3892 /label=BBa_I2032 stem_loop 3912..3955 /label=stem_loop misc_feature 3901..3980 /label=BBa_B0010 terminator 3901..3980 /label=BBa_B0010 stem_loop 3996..4015 /label=T7 TE misc_feature 4022..4022 /label=stop polyA_site 4016..4029 /label=polya misc_feature 3989..4029 /label=BBa_B0012 terminator 3989..4029 /label=BBa_B0012 promoter 4038..4057 /label=BBa_R0183 fragment misc_feature 4057..4081 /label=lacI binding site misc_feature 4068..4068 /label=Center of binding site symmetry promoter 4038..4081 /label=BBa_R0184 RBS 4090..4102 /label=RBS-3\Weak RBS 4096..4099 /label=RBS misc_feature 4090..4102 /label=BBa_B0032 RBS 4090..4102 /label=BBa_B0032 CDS 4109..4828 /label=GFP protein CDS 4109..4828 /label=BBa_E0040 terminator 4837..4884 /label=BBa_B0016 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagatg aacacgatta acatcgctaa 1261 gaacgacttc tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta 1321 cggtgagcgt ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga 1381 agcacgcttc cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc 1441 tgccgccaag cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg 1501 gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga 1561 aatcaagccg gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag 1621 tgctgacaat acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga 1681 ggctcgcttc ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga 1741 acaactcaac aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc 1801 tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga 1861 ctctattcat gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag 1921 cttacaccgc caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc 1981 tgaatacgct gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt 2041 ccaaccttgc gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc 2101 taacggtcgt cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta 2161 cgaagacgtt tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg 2221 gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc 2281 ggtcgaggac atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga 2341 catgaatcct gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga 2401 caaggctcgc aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt 2461 tgctaaccat aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc 2521 tgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa 2581 aggtaaacca atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc 2641 gggtgtcgat aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa 2701 catcatggct tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc 2761 gttctgcttc cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta 2821 taactgctcc cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc 2881 gatgctccga gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca 2941 ggacatctac gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa 3001 tgggaccgat aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa 3061 agtcaagctg ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag 3121 tgtgactaag cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca 3181 acaagtgctg gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac 3241 tcagccgaat caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac 3301 ggtggtagct gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc 3361 tgaggtcaaa gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt 3421 aactcctgat ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa 3481 cctgatgttc ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga 3541 gattgatgca cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg 3601 tagccacctt cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc 3661 actgattcac gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt 3721 gcgcgaaact atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca 3781 gttcgctgac cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg 3841 taacttgaac ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaat aatactagag 3901 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 3961 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 4021 gcgtttatat actagagtca tacgactcac tataggggaa ttgtgagcgg ataacaattc 4081 ctactagagt cacacaggaa agtactagat gcgtaaagga gaagaacttt tcactggagt 4141 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg 4201 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg 4261 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt 4321 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg 4381 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga 4441 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa 4501 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata 4561 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat 4621 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg 4681 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc 4741 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca 4801 tggcatggat gaactataca aataataata ctagagctag cataacccct tggggcctct 4861 aaacgggtct tgaggggttt tttg //