BBa_K258004
1
BBa_K258004
Keratinocyte growth factor (KGF)
2009-10-03T11:00:00Z
2015-05-08T01:11:42Z
Gene was synthesized at GENEART.
Keratinocyte growth factor (KGF) is a locally acting epithelial mitogen that is produced by cells of mesenchymal origin and has an important role in protecting and repairing epithelial tissues.Use of recombinant human KGF (palifermin) in patients with hematologic malignancies reduces the incidence and duration of severe oral mucositis experienced after intensive chemoradiotherapy. These results suggest that KGF may be useful in the treatment of patients with other kinds of tumors, including those of epithelial origin.[1]The potential effect of KGF on wound healing was assessed in vitro by measuring randomized migration and plasminogen activator (PA) activity of keratinocytes in response to the growth factor. Incubation of normal human keratinocytes with KGF in modified MCDB 153 medium significantly stimulated cell migration[2]
The Keratinocyte Growth Factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium.[3]
. KGF is weakly expressed in human skin, but is strongly upregulated in dermal fibroblasts after skin injury. Its binding to a transmembrane receptor on keratinocytes induces proliferation and migration of these cells. Furthermore, KGF has been shown to protect epithelial cells from the toxic effects of reactive oxygen species. We have identified a series of KGF-regulated genes that are likely to play a role in these processes. In addition to KGF, activin seems to be a novel player in wound healing.[4]
false
false
_358_
0
4260
9
It's complicated
true
The gene is in pMA vector having biobrick restriction sites(Ecor1, Xba1 and Spe1,Pst1,respectively)
false
Ozkan IS
BBa_S04285
1
BBa_S04285
K258004:K258001
2009-10-08T11:00:00Z
2015-05-08T01:14:37Z
false
false
_9_
0
4260
9
Not in stock
false
false
Cihan Tastan
component2032909
1
BBa_K258004
component2032910
1
BBa_K258001
annotation2032909
1
BBa_K258004
range2032909
1
1
492
annotation2032910
1
BBa_K258001
range2032910
1
501
1028
BBa_K258001
1
BBa_K258001
Lipase ABC transporter recognition domain (LARD 1)
2009-10-02T11:00:00Z
2015-05-08T01:11:42Z
LARD 1 composed of residues 303-476 of thermostable lipase (TliA) of Pseudomonas fluorescens.
Lard 1 was designed for the secretion of fusion proteins.The LARD 1 included four glycine-rich repeats comprising a β-roll structure, and were added to the C-terminus of test proteins. Either Pro-Gly linker or Factor Xa site can be added between fusion proteins and LARD 1. Upon supplementation of E. coli with ABC transporter from E. chrysanthemi (ABC transporter PrtDEF), which function well in the phylogenetically-neighboring genus E.coli, EGF-LARDs were excreted into the culture supernatant.
false
false
_358_
0
4260
9
It's complicated
true
In our designed LARD 1, we favoured not to add these cleavage site so if you want, yo can add either Pro-Gly linker or Factor Xa site. Secretion of fusion proteins with LARDs could be detected by Western blotting. Fusion proteins were traced in the culture supernatant using antibody against LARDs. Polyclonal antibodies against the C-terminal signal sequence were produced with a synthetic peptide (YQPTDRLVFQGADGST, residues 421???436 of TliA ,also of LARD).
false
Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
BBa_K258004_sequence
1
atgtgtaacgacatgacccctgagcaaatggcaacaaacgtgaactgtagtagtcctgaacgccacactcgctcttatgattatatggagggcggggatattcgtgttcgtcgcctgttctgccgtactcaatggtatctgcgcatcgataaacgtggtaaagtgaaaggcacccaagaaatgaaaaacaactataacatcatggaaatccgtaccgtggcagttggtattgtagcgatcaaaggcgtggaatccgagttctatctggcaatgaacaaagagggcaaactgtatgccaaaaaagagtgtaacgaggactgtaacttcaaagaactgatcctggagaaccattataacacctatgccagcgccaaatggacccataacggtggtgaaatgttcgtggccctgaaccaaaaaggcattccggtccgtggcaaaaaaacgaaaaaagagcagaaaaccgcccacttcctgcctatggccattaca
BBa_S04285_sequence
1
atgtgtaacgacatgacccctgagcaaatggcaacaaacgtgaactgtagtagtcctgaacgccacactcgctcttatgattatatggagggcggggatattcgtgttcgtcgcctgttctgccgtactcaatggtatctgcgcatcgataaacgtggtaaagtgaaaggcacccaagaaatgaaaaacaactataacatcatggaaatccgtaccgtggcagttggtattgtagcgatcaaaggcgtggaatccgagttctatctggcaatgaacaaagagggcaaactgtatgccaaaaaagagtgtaacgaggactgtaacttcaaagaactgatcctggagaaccattataacacctatgccagcgccaaatggacccataacggtggtgaaatgttcgtggccctgaaccaaaaaggcattccggtccgtggcaaaaaaacgaaaaaagagcagaaaaccgcccacttcctgcctatggccattacatactagagagcattgccaacctgtccacatgggtgtcacatctgccttcagcctatggtgatggtatgactcgtgtgctggaatctggcttttatgagcaaatgactcgtgatagtacgattattgtcgctaacctgtctgatccggctcgtgccaacacttgggttcaagacctgaaccgtaatgctgaacctcatacgggtaacacctttatcattgggtccgatgggaacgatctgattcaaggcggcaaaggagcggatttcatcgagggtggaaaaggcaacgacaccatccgtgacaattctgggcataacacgtttctgttttcgggccattttggtcaggatcgtatcatcggctatcagccgaccgatcgtctggtgtttcaaggtgctgatggttcaaccgatctgcgtgatcatgccaaagcagttggtgccgacacagttctgtcttttggagctgactcagtgactctggtgggagttggtctgggtggtctgtggtctgagggtgtactgattagctactag
BBa_K258001_sequence
1
agcattgccaacctgtccacatgggtgtcacatctgccttcagcctatggtgatggtatgactcgtgtgctggaatctggcttttatgagcaaatgactcgtgatagtacgattattgtcgctaacctgtctgatccggctcgtgccaacacttgggttcaagacctgaaccgtaatgctgaacctcatacgggtaacacctttatcattgggtccgatgggaacgatctgattcaaggcggcaaaggagcggatttcatcgagggtggaaaaggcaacgacaccatccgtgacaattctgggcataacacgtttctgttttcgggccattttggtcaggatcgtatcatcggctatcagccgaccgatcgtctggtgtttcaaggtgctgatggttcaaccgatctgcgtgatcatgccaaagcagttggtgccgacacagttctgtcttttggagctgactcagtgactctggtgggagttggtctgggtggtctgtggtctgagggtgtactgattagctactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z