LOCUS       BBa_S04348              2821 bp    DNA     linear       01-Jun-2024
DEFINITION  S04345:J31006
ACCESSION   BBa_S04348
VERSION     BBa_S04348.1  
FEATURES             Location/Qualifiers
     misc_feature    86..91
                     /label=-35 Region
     misc_feature    75..93
                     /label=LasR Binding Site
     misc_feature    113..118
                     /label= -10 Region
     misc_feature    125..125
                     /label=Primary Transcriptional Start
     promoter        1..126
                     /label=BBa_K091117
     misc_feature    139..142
                     /label=conserved
     RBS             135..146
                     /label=BBa_B0034
     misc_feature    1..146
                     /label=BBa_S04340
     misc_feature    153..154
                     /label=prefix
     misc_feature    326..326
                     /label=T
     misc_feature    644..644
                     /label=A
     CDS             153..902
                     /label=luxR
     misc_feature    153..908
                     /label=BBa_C0062
     misc_feature    909..933
                     /label=Help:Barcodes
     CDS             153..908
                     /label=BBa_C0062
     misc_feature    1..933
                     /label=BBa_S04341
     misc_feature    946..949
                     /label=conserved
     RBS             942..953
                     /label=BBa_B0034
     misc_feature    1..953
                     /label=BBa_S04343
     misc_feature    1539..1570
                     /label=LVA
     CDS             960..1538
                     /label=luxI
     misc_feature    960..1577
                     /label=BBa_C0061
     misc_feature    1578..1602
                     /label=Help:Barcodes
     CDS             960..1577
                     /label=BBa_C0061
     misc_feature    1..1602
                     /label=BBa_S04344
     misc_feature    1615..1618
                     /label=conserved
     RBS             1611..1622
                     /label=BBa_B0034
     misc_feature    1..1622
                     /label=BBa_S04345
     CDS             1631..2821
                     /label=tetA(C) backwards
     misc_feature    1631..1633
                     /label=stop
     primer_bind     1631..1650
                     /label=Rev primer J31006
     primer_bind     2798..2821
                     /label=Fwd primer J31006
     CDS             1631..2821
                     /label=BBa_J31006
ORIGIN
        1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt
       61 ccgggttcac cgaaatctat ctcatttgct agttataaaa ttatgaaatt tgtataaatt
      121 cttcagtact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat
      181 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat
      241 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt
      301 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt
      361 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt
      421 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa
      481 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta
      541 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt
      601 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc
      661 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat
      721 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca
      781 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc
      841 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa
      901 attaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga
      961 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta
     1021 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa
     1081 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg
     1141 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga
     1201 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg
     1261 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa
     1321 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat
     1381 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc
     1441 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta
     1501 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg
     1561 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga
     1621 aatactagag ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
     1681 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
     1741 cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg
     1801 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     1861 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     1921 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga
     1981 taatggcctg cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga
     2041 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     2101 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     2161 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     2221 tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca
     2281 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
     2341 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     2401 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     2461 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     2521 cgtagaggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa
     2581 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg
     2641 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga
     2701 tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta
     2761 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     2821 t
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