LOCUS       BBa_S04356              2077 bp    DNA     linear       01-Jun-2024
DEFINITION  J31006:S04355
ACCESSION   BBa_S04356
VERSION     BBa_S04356.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) backwards
     misc_feature    1..3
                     /label=stop
     primer_bind     1..20
                     /label=Rev primer J31006
     primer_bind     1168..1191
                     /label=Fwd primer J31006
     CDS             1..1191
                     /label=BBa_J31006
     misc_feature    1204..1207
                     /label=conserved
     RBS             1200..1211
                     /label=BBa_B0034
     CDS             1218..1940
                     /label=BBa_E0020
     stem_loop       1960..2003
                     /label=stem_loop
     misc_feature    1949..2028
                     /label=BBa_B0010
     terminator      1949..2028
                     /label=BBa_B0010
     stem_loop       2044..2063
                     /label=T7 TE
     misc_feature    2070..2070
                     /label=stop
     polyA_site      2064..2077
                     /label=polya
     misc_feature    2037..2077
                     /label=BBa_B0012
     terminator      2037..2077
                     /label=BBa_B0012
ORIGIN
        1 ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
       61 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      121 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      181 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      241 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      301 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      361 cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa
      421 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
      481 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
      541 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      601 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
      661 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      721 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      781 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      841 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      901 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      961 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1021 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1081 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1141 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca ttactagaga
     1201 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1261 tcctggtcga gctggacggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1321 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1381 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct
     1441 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1501 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1561 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1621 gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg
     1681 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
     1741 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1801 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1861 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1921 acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1981 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2041 actggctcac cttcgggtgg gcctttctgc gtttata
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