LOCUS       BBa_S04357              2097 bp    DNA     linear       01-Jun-2024
DEFINITION  B0034:S04356
ACCESSION   BBa_S04357
VERSION     BBa_S04357.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             21..1211
                     /label=tetA(C) backwards
     misc_feature    21..23
                     /label=stop
     primer_bind     21..40
                     /label=Rev primer J31006
     primer_bind     1188..1211
                     /label=Fwd primer J31006
     CDS             21..1211
                     /label=BBa_J31006
     misc_feature    1224..1227
                     /label=conserved
     RBS             1220..1231
                     /label=BBa_B0034
     CDS             1238..1960
                     /label=BBa_E0020
     stem_loop       1980..2023
                     /label=stem_loop
     misc_feature    1969..2048
                     /label=BBa_B0010
     terminator      1969..2048
                     /label=BBa_B0010
     stem_loop       2064..2083
                     /label=T7 TE
     misc_feature    2090..2090
                     /label=stop
     polyA_site      2084..2097
                     /label=polya
     misc_feature    2057..2097
                     /label=BBa_B0012
     terminator      2057..2097
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagag ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac
       61 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
      121 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
      181 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
      241 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
      301 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
      361 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcggggcc agtgacgaag
      421 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
      481 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
      541 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
      601 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc
      661 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
      721 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
      781 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
      841 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
      901 atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
      961 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
     1021 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
     1081 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
     1141 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
     1201 ttagatttca ttactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct
     1261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
     1321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
     1441 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
     1681 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     1741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1921 cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa
     1981 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2041 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
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