LOCUS       BBa_S04389              2911 bp    DNA     linear       01-Jun-2024
DEFINITION  S04388:J31006
ACCESSION   BBa_S04389
VERSION     BBa_S04389.1  
FEATURES             Location/Qualifiers
     misc_feature    75..93
                     /label=LasR
     misc_feature    94..113
                     /label=LuxR
     misc_feature    113..118
                     /label=-10
     misc_feature    86..91
                     /label=-35
     misc_feature    125..125
                     /label=Transcriptional Start
     promoter        1..126
                     /label=BBa_K091146
     misc_feature    139..142
                     /label=conserved
     RBS             135..146
                     /label=BBa_B0034
     misc_feature    1..146
                     /label=BBa_S04384
     CDS             153..875
                     /label=cinR
     misc_feature    876..908
                     /label=LVA
     misc_feature    915..939
                     /label=Help:Barcodes
     CDS             153..939
                     /label=BBa_C0077
     misc_feature    1..939
                     /label=BBa_S04385
     misc_feature    952..955
                     /label=conserved
     RBS             948..959
                     /label=BBa_B0034
     misc_feature    1..959
                     /label=BBa_S04386
     CDS             966..1628
                     /label=CinI
     misc_feature    1629..1661
                     /label=LVA
     misc_feature    1668..1692
                     /label=Help:Barcodes
     CDS             966..1692
                     /label=BBa_C0076
     misc_feature    1..1692
                     /label=BBa_S04387
     misc_feature    1705..1708
                     /label=conserved
     RBS             1701..1712
                     /label=BBa_B0034
     misc_feature    1..1712
                     /label=BBa_S04388
     CDS             1721..2911
                     /label=tetA(C) backwards
     misc_feature    1721..1723
                     /label=stop
     primer_bind     1721..1740
                     /label=Rev primer J31006
     primer_bind     2888..2911
                     /label=Fwd primer J31006
     CDS             1721..2911
                     /label=BBa_J31006
ORIGIN
        1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt
       61 ccgggttcac cgaaatctat ctcatttgct agtacctgta ggatcgtaca ggtataaatt
      121 cttcagtact agagaaagag gagaaatact agatgattga gaatacctat agcgaaaagt
      181 tcgagtccgc gttcgaacag atcaaggcgg cggccaacgt ggatgccgcc atccgtattc
      241 tccaggcgga atataacctc gatttcgtca cctaccatct cgcccagacg atcgcgagca
      301 agatcgattc gcccttcgtg cgcaccacct atccggatgc ctgggtttcc cgctacctcc
      361 tcaacagcta tgtgaaggtc gatccgatcg tcaagcaggg cttcgaacgc cagctgccct
      421 tcgactggag cgaggtcgaa ccgacgccgg aggcctatgc catgctggtc gacgcccaga
      481 aacacggcat cggtggcaat ggctactcca tccccgtcgc cgacaaggcg cagcgccgcg
      541 ccctgctgtc gctgaatgcc cgtataccgg ccgacgaatg gaccgagctc gtgcgccgct
      601 gccgcaacga gtggatcgag atcgcccatc tgatccaccg caaggccgtc tatgagctgc
      661 atggcgaaaa cgatccggtg ccggcattgt cgccgcgcga gatcgagtgt ctgcactgga
      721 ccgccctcgg caaggattac aaggatattt cggtcatcct gggcatatca gagcatacca
      781 cacgcgatta cctgaagacc gcccgcttca agctcggctg cgccacgatc tcggccgccg
      841 cgtcgcgggc tgttcaattg cgcatcatca atcccgctgc aaacgacgaa aactacgctt
      901 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagaaa gaggagaaat
      961 actagatgtt cgttatcatc caggctcacg aataccagaa atacgctgct gttctggacc
     1021 agatgttccg tctgcgtaaa aaagttttcg ctgacaccct gtgctgggac gttccggtta
     1081 tcggtccgta cgaacgtgac tcctacgact ccctggctcc ggcttacctg gtttggtgca
     1141 acgactcccg tacccgtctg tacggtggta tgcgtctgat gccgaccacc ggtccgaccc
     1201 tgctgtacga cgttttccgt gaaaccttcc cggacgctgc tgacctgatc gctccgggta
     1261 tctgggaagg tacccgtatg tgcatcgacg aagaagctat cgctaaagac ttcccggaaa
     1321 tcgacgctgg tcgtgctttc tccatgatgc tgctggctct gtgcgaatgc gctctggacc
     1381 acggtatcca caccatgatc tccaactacg aaccgtacct gaaacgtgtt tacaaacgtg
     1441 ctggtgctga agttgaagaa ctgggtcgtg ctgacggtta cggtaaatac ccggtttgct
     1501 gcggtgcttt cgaagtttcc gaccgtgttc tgcgtaaaat gcgtgctgct ctgggtctga
     1561 ccctgccgct gtacgttcgt cacgttccgg ctcgttccgt tgttacccag ttcctggaaa
     1621 tggctgctgc tgctaacgac gaaaactacg ctctggttgc ttaataaccc tgatagtgct
     1681 agtgtagatc cctactagag aaagaggaga aatactagag ttaggtcgag gtggcccggc
     1741 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc
     1801 atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc
     1861 caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt
     1921 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
     1981 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
     2041 agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     2101 tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     2161 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     2221 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     2281 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     2341 gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     2401 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     2461 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     2521 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     2581 cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg
     2641 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc
     2701 caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata
     2761 gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga
     2821 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     2881 ggatgacgat gagcgcattg ttagatttca t
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