LOCUS BBa_B0015 129 bp DNA linear 01-Jun-2024 DEFINITION double terminator (B0010-B0012) ACCESSION BBa_B0015 VERSION BBa_B0015.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttata // LOCUS BBa_S04402 3819 bp DNA linear 01-Jun-2024 DEFINITION S04401:S04353 ACCESSION BBa_S04402 VERSION BBa_S04402.1 FEATURES Location/Qualifiers RBS 226..231 /label=embedded RBS stem_loop 35..74 /label=stem_loop stem_loop 91..136 /label=stem_loop promoter 1..220 /label=unidentified/uncharacterized CinR dependent promoter 1..231 /label=BBa_R0077 misc_feature 244..247 /label=conserved RBS 240..251 /label=BBa_B0034 misc_feature 1..251 /label=BBa_S04394 CDS 258..974 /label=lasR misc_feature 975..1007 /label=LVA misc_feature 737..737 /label=G misc_feature 1014..1038 /label=Help:Barcodes CDS 258..1038 /label=BBa_C0079 misc_feature 1..1038 /label=BBa_S04395 misc_feature 1051..1054 /label=conserved RBS 1047..1058 /label=BBa_B0034 misc_feature 1..1058 /label=BBa_S04396 CDS 1065..1667 /label=LasI misc_feature 1668..1700 /label=LVA misc_feature 1707..1731 /label=Help:Barcodes CDS 1065..1731 /label=BBa_C0078 misc_feature 1..1731 /label=BBa_S04397 misc_feature 1744..1747 /label=conserved RBS 1740..1751 /label=BBa_B0034 misc_feature 1..1751 /label=BBa_S04398 CDS 1760..2950 /label=tetA(C) backwards misc_feature 1760..1762 /label=stop primer_bind 1760..1779 /label=Rev primer J31006 primer_bind 2927..2950 /label=Fwd primer J31006 CDS 1760..2950 /label=BBa_J31006 misc_feature 1..2950 /label=BBa_S04399 misc_feature 2963..2966 /label=conserved RBS 2959..2970 /label=BBa_B0034 misc_feature 1..2970 /label=BBa_S04400 CDS 2977..3651 /label=mrfp1 misc_feature 3658..3682 /label=Help:Barcodes CDS 2977..3682 /label=BBa_E1010 misc_feature 1..3682 /label=BBa_S04401 stem_loop 3702..3745 /label=stem_loop misc_feature 3691..3770 /label=BBa_B0010 terminator 3691..3770 /label=BBa_B0010 stem_loop 3786..3805 /label=T7 TE misc_feature 3812..3812 /label=stop polyA_site 3806..3819 /label=polya misc_feature 3779..3819 /label=BBa_B0012 terminator 3779..3819 /label=BBa_B0012 misc_feature 3691..3819 /label=BBa_S04353 ORIGIN 1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc 61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac 121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga 181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctggag gtactagaga 241 aagaggagaa atactagatg gccttggttg acggttttct tgagctggaa cgctcaagtg 301 gaaaattgga gtggagcgcc atcctccaga agatggcgag cgaccttgga ttctcgaaga 361 tcctgttcgg cctgttgcct aaggacagcc aggactacga gaacgccttc atcgtcggca 421 actacccggc cgcctggcgc gagcattacg accgggctgg ctacgcgcgg gtcgacccga 481 cggtcagtca ctgtacccag agcgtactgc cgattttctg ggaaccgtcc atctaccaga 541 cgcgaaagca gcacgagttc ttcgaggaag cctcggccgc cggcctggtg tatgggctga 601 ccatgccgct gcatggtgct cgcggcgaac tcggcgcgct gagcctcagc gtggaagcgg 661 aaaaccgggc cgaggccaac cgtttcatag agtcggtcct gccgaccctg tggatgctca 721 aggactacgc actgcaaagc ggtgccggac tggccttcga acatccggtc agcaaaccgg 781 tggttctgac cagccgggag aaggaagtgt tgcagtggtg cgccatcggc aagaccagtt 841 gggagatatc ggttatctgc aactgctcgg aagccaatgt gaacttccat atgggaaata 901 ttcggcggaa gttcggtgtg acctcccgcc gcgtagcggc cattatggcc gttaatttgg 961 gtcttattac tctcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1021 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatgatc gttcagatcg 1081 gtcgtcgtga agagttcgac aaaaaactgc tgggtgaaat gcacaaactg cgtgctcagg 1141 ttttcaaaga acgtaaaggt tgggacgttt ccgttatcga cgaaatggaa atcgacggtt 1201 acgacgctct gtccccgtac tacatgctga tccaggaaga caccccggaa gctcaggttt 1261 tcggttgctg gcgtatcttc gacaccaccg gtccgtacat gctgaaaaac accttcccgg 1321 aactgctgca cggtaaagaa gctccgtgct ccccgcacat ctgggaactg tcccgtttcg 1381 ctatcaactc cggtcagaaa ggttccctgg gtttctccga ctgcaccctg gaagctatgc 1441 gtgctctggc tcgttactcc ttgcagaacg acatccagac cctggttacc gttaccaccg 1501 ttggtgttga aaaaatgatg atccgtgctg gtctggacgt ttcccgtttc ggtccgcacc 1561 tgaaaatcgg tatcgaacgt gctgttgctc tgcgtatcga actgaacgct aaaacccaga 1621 tcgctctgta cggtggtgtt ctggttgaac agcgtctggc tgtttccgct gctaacgacg 1681 aaaactacgc tctggttgct taataactct gatagtgcta gtgtagatct ctactagaga 1741 aagaggagaa atactagagt taggtcgagg tggcccggct ccatgcaccg cgacgcaacg 1801 cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc 1861 tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg 1921 taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca 1981 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga 2041 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca 2101 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcggggcca gtgacgaagg 2161 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc 2221 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga 2281 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc 2341 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg 2401 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa 2461 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca 2521 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg 2581 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga 2641 tgcgtccggc gtagaggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag 2701 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc 2761 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt 2821 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa 2881 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt 2941 tagatttcat tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc 3001 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 3061 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 3121 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 3181 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 3241 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 3301 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 3361 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 3421 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 3481 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 3541 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac 3601 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc 3661 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 3721 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3781 acactggctc accttcgggt gggcctttct gcgtttata //