LOCUS       BBa_S04444               991 bp    DNA     linear       10-May-2024
DEFINITION  S04438:E5500
ACCESSION   BBa_S04444
VERSION     BBa_S04444.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..242
                     /label=BBa_J61046
     RBS             251..265
                     /label=RBS-1\Strong
     RBS             258..262
                     /label=RBS
     misc_feature    251..265
                     /label=BBa_B0030
     RBS             251..265
                     /label=BBa_B0030
     CDS             272..991
                     /label=GFP protein
     CDS             272..991
                     /label=BBa_E0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat aacttcgtat aatgtatgct atacgaagtt
      241 attactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
      301 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      361 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      421 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      481 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      541 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      601 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      661 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      721 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      781 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      841 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      901 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      961 acatggcatg gatgaactat acaaataata a
//