BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_S04524
1
BBa_S04524
K361000:B0014
2010-10-23T11:00:00Z
2015-05-08T01:14:41Z
false
false
_9_
0
6123
9
It's complicated
false
false
Jacky, Fong Chuen, Loo
component2095183
1
BBa_K361000
component2095190
1
BBa_B0014
annotation2095183
1
BBa_K361000
range2095183
1
1
1155
annotation2095190
1
BBa_B0014
range2095190
1
1164
1258
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K361000
1
Rci
Rci site-specific recombinase
2010-10-23T11:00:00Z
2015-05-08T01:12:12Z
It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1
This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition.
After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.
false
true
_476_
0
6123
9
It's complicated
true
The gene is come from muliple inversion systems in R64 plasmid and there is no further modification.
Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process.
false
Jacky, Fong Chuen, Loo
annotation2095181
1
Rci recombinase
range2095181
1
1
1155
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K361000_sequence
1
atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_S04524_sequence
1
atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z