LOCUS       BBa_S04547              2470 bp    DNA     linear       14-Jun-2024
DEFINITION  K208016:K116634
ACCESSION   BBa_S04547
VERSION     BBa_S04547.1  
FEATURES             Location/Qualifiers
     CDS             1..718
                     /label=GFP fusion brick
     misc_feature    1..1
                     /label=deletion of TG
     CDS             1..718
                     /label=BBa_K125500
     stem_loop       738..781
                     /label=stem_loop
     misc_feature    727..806
                     /label=BBa_B0010
     terminator      727..806
                     /label=BBa_B0010
     stem_loop       822..841
                     /label=T7 TE
     misc_feature    848..848
                     /label=stop
     polyA_site      842..855
                     /label=polya
     misc_feature    815..855
                     /label=BBa_B0012
     terminator      815..855
                     /label=BBa_B0012
     misc_feature    1..855
                     /label=BBa_K208016
     RBS             864..876
                     /label=RBS-3\Weak
     RBS             870..873
                     /label=RBS
     misc_feature    864..876
                     /label=BBa_B0032
     RBS             864..876
                     /label=BBa_B0032
     misc_feature    883..884
                     /label=prefix
     misc_feature    1056..1056
                     /label=T
     misc_feature    1374..1374
                     /label=A
     CDS             883..1632
                     /label=luxR
     misc_feature    883..1638
                     /label=BBa_C0062
     misc_feature    1639..1663
                     /label=Help:Barcodes
     CDS             883..1638
                     /label=BBa_C0062
     misc_feature    864..1663
                     /label=BBa_K116616
     RBS             1672..1684
                     /label=RBS-3\Weak
     RBS             1678..1681
                     /label=RBS
     misc_feature    1672..1684
                     /label=BBa_B0032
     RBS             1672..1684
                     /label=BBa_B0032
     misc_feature    2270..2301
                     /label=LVA
     CDS             1691..2269
                     /label=luxI
     misc_feature    1691..2308
                     /label=BBa_C0061
     misc_feature    2309..2333
                     /label=Help:Barcodes
     CDS             1691..2308
                     /label=BBa_C0061
     stem_loop       2353..2396
                     /label=stem_loop
     misc_feature    2342..2421
                     /label=BBa_B0010
     terminator      2342..2421
                     /label=BBa_B0010
     stem_loop       2437..2456
                     /label=T7 TE
     misc_feature    2463..2463
                     /label=stop
     polyA_site      2457..2470
                     /label=polya
     misc_feature    2430..2470
                     /label=BBa_B0012
     terminator      2430..2470
                     /label=BBa_B0012
     terminator      2342..2470
                     /label=BBa_B0015
     misc_feature    1691..2470
                     /label=BBa_K116615
     misc_feature    1672..2470
                     /label=BBa_K116625
     misc_feature    864..2470
                     /label=BBa_K116634
ORIGIN
        1 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
       61 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      121 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      181 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
      241 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      301 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      361 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      421 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
      481 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
      541 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      601 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      661 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatta
      721 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      781 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      841 ctttctgcgt ttatatacta gagtcacaca ggaaagtact agatgaaaaa cataaatgcc
      901 gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat
      961 caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt
     1021 tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg
     1081 aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac
     1141 tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct
     1201 ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt
     1261 catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat
     1321 atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt
     1381 gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga
     1441 gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata
     1501 ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat
     1561 acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca
     1621 tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga gtcacacagg
     1681 aaagtactag atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga
     1741 gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt
     1801 agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta
     1861 tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga
     1921 ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc
     1981 taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc
     2041 tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg
     2101 tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa
     2161 agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt
     2221 tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga
     2281 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga
     2341 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2401 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2461 tgcgtttata
//