LOCUS       BBa_S04560              2615 bp    DNA     linear       17-May-2024
DEFINITION  S04559:K112808
ACCESSION   BBa_S04560
VERSION     BBa_S04560.1  
FEATURES             Location/Qualifiers
     RBS             90..95
                     /label=SD
     misc_feature    74..79
                     /label=Biobrick-Scar-adaptation
     stem_loop       1..15
                     /label=Predicted Stem Loop
     stem_loop       16..36
                     /label=Predicted Stem Loop
     stem_loop       39..71
                     /label=Predicted Stem Loop
     stem_loop       72..96
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    1..96
                     /label=BBa_K115001
     CDS             103..822
                     /label=GFP protein
     CDS             103..822
                     /label=BBa_E0040
     misc_feature    1..822
                     /label=BBa_S04559
     CDS             847..1503
                     /label=T4 holin
     CDS             831..1506
                     /label=BBa_K112805
     CDS             1531..2025
                     /label=T4 Endolysin
     CDS             1515..2028
                     /label=BBa_K112806
     stem_loop       2048..2091
                     /label=stem_loop
     misc_feature    2037..2116
                     /label=BBa_B0010
     terminator      2037..2116
                     /label=BBa_B0010
     stem_loop       2132..2151
                     /label=T7 TE
     misc_feature    2158..2158
                     /label=stop
     polyA_site      2152..2165
                     /label=polya
     misc_feature    2125..2165
                     /label=BBa_B0012
     terminator      2125..2165
                     /label=BBa_B0012
     promoter        2174..2208
                     /label=BBa_J23116
     CDS             2231..2524
                     /label=T4 antiholin
     CDS             2217..2527
                     /label=BBa_K112807
     stem_loop       2547..2590
                     /label=stem_loop
     misc_feature    2536..2615
                     /label=BBa_B0010
     terminator      2536..2615
                     /label=BBa_B0010
     misc_feature    831..2615
                     /label=BBa_K112808
ORIGIN
        1 gccgcgacaa gcggtccggg cgccctaggg gcccggcgga gacgggcgcc ggaggtgtcc
       61 gacgcctgct cgtcaagttc ttgctccttg gaggattact agatgcgtaa aggagaagaa
      121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      241 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      301 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      361 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      421 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      481 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      541 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      601 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      661 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
      721 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      781 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag cttaaaagga
      841 gggtctatgg cagcacctag aatatcattt tcgccctctg atattctatt tggtgttctc
      901 gatcgcttgt tcaaagataa cgctaccggg aaggttcttg cttcccgggt agctgtcgta
      961 attcttttgt ttataatggc gattgtttgg tataggggag atagtttctt tgagtactat
     1021 aagcaatcaa agtatgaaac atacagtgaa attattgaaa aggaaagaac tgcacgcttt
     1081 gaatctgtcg ccctggaaca actccagata gttcatatat catctgaggc agactttagt
     1141 gcggtgtatt ctttccgccc taaaaactta aactattttg ttgatattat agcatacgaa
     1201 ggaaaattac cttcaacaat aagtgaaaaa tcacttggag gatatcctgt tgataaaact
     1261 atggatgaat atacagttca tttaaatgga cgtcattatt attccaactc aaaatttgct
     1321 tttttaccaa ctaaaaagcc tactcccgaa ataaactaca tgtacagttg tccatatttt
     1381 aatttggata atatctatgc tggaacgata accatgtact ggtatagaaa tgatcatata
     1441 agtaatgacc gccttgaatc aatatgtgct caggcggcca gaatattagg aagggctaaa
     1501 taattatact agagatactt aggaggtatt atgaatatat ttgaaatgtt acgtatagat
     1561 gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt
     1621 catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt
     1681 gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat
     1741 gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct
     1801 cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt
     1861 gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca
     1921 gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt
     1981 acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta ctagagccag
     2041 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2101 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2161 ttatatacta gagttgacag ctagctcagt cctagggact atgctagcta ctagaggata
     2221 ggaggccttt atggccttaa aagcaacagc actttttgcc atgctaggat tgtcatttgt
     2281 tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta tggaatctgg
     2341 tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg aacaattcta
     2401 tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg aatgtataga
     2461 gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat tggagactga
     2521 atgaaattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2581 ttcgttttat ctgttgtttg tcggtgaacg ctctc
//