BBa_K531004
1
BBa_K531004
dspB from Actinobacillus actinomycetemcomitansis optimized for Caulobacter crescentus
2011-07-10T11:00:00Z
2015-05-08T01:12:36Z
Kaplan, J., Ragunath, C., Ramasubbu, N., and Fine, D. Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous beta-Hexosaminidase Activity. Journal of Bacteriology. 2003 August; 185(16): 4693-4698.
DspB comes from the bacteria Actinobacillus actinomycetemcomitansis. It is most likely an N-acetylglucosaminidase that causes the detachment of cells from biofilm colonies through the 1→ 4 glycosidic bond of β-substituted N-acetylglucosamine. DspB is homologous to family 20 glycosyl hydrolases which cleave terminal monosaccharide residues.
false
false
_697_
0
9994
9
Not in stock
false
The WT gene has a low GC content ~40%. Caulobacter crescentus is GC rich and prefers a high GC content. Therefore, we optimized the GC content by comparing the codons in the wild type dspB gene to their corresponding amino acid codons that are most expressed in Caulobacter.
We also eliminated the stop codon at the end of dspB because we wanted to add another gene to it that also needed to be transcribed.
false
Nora Kostow
BBa_S04611
1
BBa_S04611
K531004:K531001
2011-09-20T11:00:00Z
2015-05-08T01:14:42Z
false
false
_9_
0
8840
9
It's complicated
false
false
Alexander Aaring
component2170239
1
BBa_K531004
component2170240
1
BBa_K531001
annotation2170239
1
BBa_K531004
range2170239
1
1
1085
annotation2170240
1
BBa_K531001
range2170240
1
1094
1453
BBa_K531001
1
BBa_K531001
<i>C. crescentus</i> S-layer RsaA C-term
2011-07-03T11:00:00Z
2015-05-08T01:12:36Z
This comes from genomic ''Caulobacter'' CB15N.
This is a secretion tag for secretion via a Type I secretion pathway in ''Caulobacter crescentus''. It should be appended to the C-terminal end of another protein to act as a secretion tag.
false
false
_697_
0
8840
9
In stock
true
Using the Registry's suggested primer combinations results in the formation of an in sequence stop codon between two coding regions. A one nucleotide change was necessary in both the prefix of this coding sequence and the suffix of the coding region to be added in front of this gene to ensure that their is no stop codon in sequence.
false
Alexander Aaring
BBa_S04611_sequence
1
tgaactgctgcgtgaagggcaactccatctatccgcagaagacctccaccaagcagaccggcctcatgctggatatcgcccgccacttctactcgccggaagtgatcaagtcgttcatcgacaccatctcgctgagcggcggcaacttcctgcacctgcacttctccgaccatgaaaactatgcgatcgagtcgcacctcctcaaccagcgcgcggaaaacgccgtccagggcaaggacggcatctacatcaacccgtacaccggcaagccgttcctgagctatcgccagctggacgacatcaaggcgtatgcgaaggccaaggggatcgagctgatccccgagctggacagccccaaccacatgaccgccatcttcaagctggtccagaaggaccgcggtgtcaagtacctccagggcctcaagagccgccaggtcgacgacgagatcgacatcaccaacgcggactcgatcacgttcatgcagtccctgatgtcggaggtcatcgacatcttcggggacacgtcgcagcatttccatatcggtggcgacgagttcggctatagcgtcgagagcaaccatgagttcatcacctacgccaacaagctgagctacttcctggaaaagaagggcctgaagacccgcatgtggaacgatggcctgatcaagaatacctttgaacagatcaatcccaacatcgagatcacgtactggagctatgatggcgacacgcaagacaagaacgaggcggccgaacgccgcgacatgcgggtctcgctgccggagctgctggccaagggtttcaccgtgctcaattacaattcctattacctgtatatcgtcccgaaggcctcgccgaccttcagccaggacgcggcctttgccgccaaggatgtcatcaagaactgggatctgggggtctgggacggccgcaataccaagaaccgggtccagaacacccatgagatcgccggcgccgccctctcgatctggggcgaggacgccaaggccctgaaggacgaaacgatccagaagaacaccaagagcctgctcgaagccgtgatccacaagaccaatggcgatgagtactagaggatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
BBa_K531001_sequence
1
gatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
BBa_K531004_sequence
1
tgaactgctgcgtgaagggcaactccatctatccgcagaagacctccaccaagcagaccggcctcatgctggatatcgcccgccacttctactcgccggaagtgatcaagtcgttcatcgacaccatctcgctgagcggcggcaacttcctgcacctgcacttctccgaccatgaaaactatgcgatcgagtcgcacctcctcaaccagcgcgcggaaaacgccgtccagggcaaggacggcatctacatcaacccgtacaccggcaagccgttcctgagctatcgccagctggacgacatcaaggcgtatgcgaaggccaaggggatcgagctgatccccgagctggacagccccaaccacatgaccgccatcttcaagctggtccagaaggaccgcggtgtcaagtacctccagggcctcaagagccgccaggtcgacgacgagatcgacatcaccaacgcggactcgatcacgttcatgcagtccctgatgtcggaggtcatcgacatcttcggggacacgtcgcagcatttccatatcggtggcgacgagttcggctatagcgtcgagagcaaccatgagttcatcacctacgccaacaagctgagctacttcctggaaaagaagggcctgaagacccgcatgtggaacgatggcctgatcaagaatacctttgaacagatcaatcccaacatcgagatcacgtactggagctatgatggcgacacgcaagacaagaacgaggcggccgaacgccgcgacatgcgggtctcgctgccggagctgctggccaagggtttcaccgtgctcaattacaattcctattacctgtatatcgtcccgaaggcctcgccgaccttcagccaggacgcggcctttgccgccaaggatgtcatcaagaactgggatctgggggtctgggacggccgcaataccaagaaccgggtccagaacacccatgagatcgccggcgccgccctctcgatctggggcgaggacgccaaggccctgaaggacgaaacgatccagaagaacaccaagagcctgctcgaagccgtgatccacaagaccaatggcgatgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z