LOCUS       BBa_S04723              2992 bp    DNA     linear       17-May-2024
DEFINITION  J176028:J176041
ACCESSION   BBa_S04723
VERSION     BBa_S04723.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     RBS             523..540
                     /label=BBa_J176012
     misc_feature    541..546
                     /label=BBa_J176041
     misc_feature    547..1005
                     /label=BBa_J176020
     misc_feature    1006..1011
                     /label=BBa_J176041
     misc_feature    1012..1545
                     /label=BBa_J176018
     misc_feature    1546..1551
                     /label=BBa_J176041
     misc_feature    1552..2952
                     /label=BBa_J176023
     misc_feature    2953..2958
                     /label=BBa_J176041
     misc_feature    2959..2992
                     /label=BBa_J176024
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacccgccgc caccatggag
      541 actagaatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag
      601 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
      661 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa
      721 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac
      781 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta
      841 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg
      901 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac
      961 aaaggtcaaa gacagttgac tgtatcgccg gaatttccgg ggatcactag agcatccaac
     1021 ttgaacattt cgagaaagct taccatatca accccatcat gctctttcga aaattcaaat
     1081 agcacatcca ttccttcgcc cgcttcctca tctcaaagcc acactccaat gagaaacatg
     1141 agctcactct ctgataacag cgttttcagc cggaatatgg aacaatcatc accaatcact
     1201 ccaagtatgt accaatttgg tcagcagcag tcaaacagta tatgtggtag caccgttagt
     1261 gtgaatagtc tggtgaatac aaataacaaa caaaggatct acgaacaaat cacgggtcct
     1321 aacagcaata acgcaaccaa tgattatatt gatttgctaa acctaaatga gtctaacaag
     1381 gaaaaccaaa atcccgcaac ggcgcattac ctcaatgggg gcccacccaa gacaagcttc
     1441 attaaccatg gaatgttccc ctcgccaact gggaccataa atagcggtaa atctagcagt
     1501 gcctcatctt taatttcttt tggtatgggc aatacccaag taataactag aatgtccgag
     1561 agggaagtgt cgactgcgcc ggcgggaaca gacatgcctg cggccaagaa gcagaagctg
     1621 agcagtgacg agaacagcaa tccagacctc tctggagacg agaatgatga cgctgtcagt
     1681 atagaaagtg gtacaaacac tgaacgccct gatacaccta caaacacgcc aaatgcacct
     1741 ggaaggaaaa gttggggaaa gggaaaatgg aagtcaaaga aatgcaaata ttctttcaaa
     1801 tgtgtaaata gtctcaagga agatcataac caaccattgt ttggagttca gtttaactgg
     1861 cacagtaaag aaggagatcc attagtgttt gcaactgtag gaagcaacag agttaccttg
     1921 tatgaatgtc attcacaagg agaaatccgg ttgttgcaat cttacgtgga tgctgatgct
     1981 gatgaaaact tttacacttg tgcatggacc tatgatagca atacgagcca tcctctgctg
     2041 gctgtagctg gatcaagagg cataattagg ataataaatc ctataacaat gcagtgtata
     2101 aagcactatg ttggccatgg aaatgctatc aatgagctga aattccatcc aagagatcca
     2161 aatcttctcc tgtcagtaag taaagatcat gctttacgat tatggaatat ccagacggac
     2221 actctggtgg caatatttgg aggcgtagaa gggcacagag atgaagttct aagtgctgat
     2281 tatgatcttt tgggtgaaaa aataatgtcc tgtggtatgg atcattctct taaactttgg
     2341 aggatcaatt caaagagaat gatgaatgca attaaggaat cttatgatta taatccaaat
     2401 aaaactaaca ggccatttat ttctcagaaa atccattttc ctgatttttc taccagagac
     2461 atacatagga attatgttga ttgtgtgcga tggttaggcg atttgatact ttctaagagt
     2521 ggccgtgcca ttttacattc ccaccagcaa tgtatgagag atccagtgtc tccgaatctt
     2581 cgccagcatt tgtcttgtga aaatgccatt gtgtgctgga aacctggcaa gatggaagat
     2641 gatatagata aaattaaacc cagtgaatct aatgtgacta ttcttgggcg atttgattac
     2701 agccagtgtg acatttggta catgaggttt tctatggatt tctggcaaaa gatgcttgca
     2761 ttgggcaatc aagttggcaa actttatgtt tgggatttag aagtagaaga tcctcataaa
     2821 gccaaatgta caacactgac tcatcataaa tgtggtgctg ctattcgaca aaccagtttt
     2881 agcagggata gcagcattct tatagctgtt tgtgatgatg ccagtatttg gcgctgggat
     2941 cgacttcgat tgactagacc caagaaaaag cgcaaggtag cgtaaagctg ag
//