LOCUS       BBa_S04724              2527 bp    DNA     linear       17-May-2024
DEFINITION  J176028:J176012
ACCESSION   BBa_S04724
VERSION     BBa_S04724.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     RBS             523..540
                     /label=BBa_J176012
     misc_feature    541..546
                     /label=BBa_J176041
     misc_feature    547..1080
                     /label=BBa_J176018
     misc_feature    1081..1086
                     /label=BBa_J176041
     misc_feature    1087..2487
                     /label=BBa_J176023
     misc_feature    2488..2493
                     /label=BBa_J176041
     misc_feature    2494..2527
                     /label=BBa_J176024
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacccgccgc caccatggag
      541 actagagcat ccaacttgaa catttcgaga aagcttacca tatcaacccc atcatgctct
      601 ttcgaaaatt caaatagcac atccattcct tcgcccgctt cctcatctca aagccacact
      661 ccaatgagaa acatgagctc actctctgat aacagcgttt tcagccggaa tatggaacaa
      721 tcatcaccaa tcactccaag tatgtaccaa tttggtcagc agcagtcaaa cagtatatgt
      781 ggtagcaccg ttagtgtgaa tagtctggtg aatacaaata acaaacaaag gatctacgaa
      841 caaatcacgg gtcctaacag caataacgca accaatgatt atattgattt gctaaaccta
      901 aatgagtcta acaaggaaaa ccaaaatccc gcaacggcgc attacctcaa tgggggccca
      961 cccaagacaa gcttcattaa ccatggaatg ttcccctcgc caactgggac cataaatagc
     1021 ggtaaatcta gcagtgcctc atctttaatt tcttttggta tgggcaatac ccaagtaata
     1081 actagaatgt ccgagaggga agtgtcgact gcgccggcgg gaacagacat gcctgcggcc
     1141 aagaagcaga agctgagcag tgacgagaac agcaatccag acctctctgg agacgagaat
     1201 gatgacgctg tcagtataga aagtggtaca aacactgaac gccctgatac acctacaaac
     1261 acgccaaatg cacctggaag gaaaagttgg ggaaagggaa aatggaagtc aaagaaatgc
     1321 aaatattctt tcaaatgtgt aaatagtctc aaggaagatc ataaccaacc attgtttgga
     1381 gttcagttta actggcacag taaagaagga gatccattag tgtttgcaac tgtaggaagc
     1441 aacagagtta ccttgtatga atgtcattca caaggagaaa tccggttgtt gcaatcttac
     1501 gtggatgctg atgctgatga aaacttttac acttgtgcat ggacctatga tagcaatacg
     1561 agccatcctc tgctggctgt agctggatca agaggcataa ttaggataat aaatcctata
     1621 acaatgcagt gtataaagca ctatgttggc catggaaatg ctatcaatga gctgaaattc
     1681 catccaagag atccaaatct tctcctgtca gtaagtaaag atcatgcttt acgattatgg
     1741 aatatccaga cggacactct ggtggcaata tttggaggcg tagaagggca cagagatgaa
     1801 gttctaagtg ctgattatga tcttttgggt gaaaaaataa tgtcctgtgg tatggatcat
     1861 tctcttaaac tttggaggat caattcaaag agaatgatga atgcaattaa ggaatcttat
     1921 gattataatc caaataaaac taacaggcca tttatttctc agaaaatcca ttttcctgat
     1981 ttttctacca gagacataca taggaattat gttgattgtg tgcgatggtt aggcgatttg
     2041 atactttcta agagtggccg tgccatttta cattcccacc agcaatgtat gagagatcca
     2101 gtgtctccga atcttcgcca gcatttgtct tgtgaaaatg ccattgtgtg ctggaaacct
     2161 ggcaagatgg aagatgatat agataaaatt aaacccagtg aatctaatgt gactattctt
     2221 gggcgatttg attacagcca gtgtgacatt tggtacatga ggttttctat ggatttctgg
     2281 caaaagatgc ttgcattggg caatcaagtt ggcaaacttt atgtttggga tttagaagta
     2341 gaagatcctc ataaagccaa atgtacaaca ctgactcatc ataaatgtgg tgctgctatt
     2401 cgacaaacca gttttagcag ggatagcagc attcttatag ctgtttgtga tgatgccagt
     2461 atttggcgct gggatcgact tcgattgact agacccaaga aaaagcgcaa ggtagcgtaa
     2521 agctgag
//