LOCUS       BBa_S05072              1080 bp    DNA     linear       01-Jun-2024
DEFINITION  B0015:P1006
ACCESSION   BBa_S05072
VERSION     BBa_S05072.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     CDS             138..1001
                     /label=ampicillin resistance
     RBS             1006..1010
                     /label=ampR RBS
     promoter        1019..1024
                     /label=ampR -10
     promoter        1045..1050
                     /label=ampR -35
     misc_feature    138..1080
                     /label=BBa_P1006
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtta ttaccaatgc ttaatcaggg aggcacctat ctcagcgatc
      181 tggcgattac gttcgtccat agttgcctgg ctccccgtcg tgtagataac tacgatacgg
      241 gagggcttac catctggccc cagggctgca ataataccgc gggacccacg ctcaccggct
      301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      361 actttatccg cctccatcca gtctattaat tgttgccggg aagcgagagt aagaagttcg
      421 ccagttaaga gtttgcgcaa cgttgttgcc atagctacag gcatcgtggt gtcacgctcg
      481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatca
      541 cccatgttgt gcaaaaaagc ggtaagctcc ttcggtcctc cgatcgttgt cagaagtaag
      601 ttggccgccg tgttatcact catggttatg gcagcgctgc ataattcgcg tactgtcatg
      661 ccgtccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacag
      781 agcagaactt taaaagtgct catcattggg aaacgttctt cggggcgaaa actctcaaga
      841 atcttaccgc tgttgaggtc cagttcgatg taacccacac gtgcacccaa ctgatcttcg
      901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      961 aaaaagggaa taagggcgac acggaaatgt tgaatactca tattcttcct ttttcaatat
     1021 tattgaagca tttatcaggg ttattgtctc atgagcggat agatatttga atgtatttag
//