LOCUS       BBa_S05321              3448 bp    DNA     linear       17-May-2024
DEFINITION  R0062:K274100
ACCESSION   BBa_S05321
VERSION     BBa_S05321.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..67
                     /label=BBa_K118012
     CDS             74..985
                     /label=BBa_I742151
     misc_feature    994..996
                     /label=SacI
     RBS             997..1003
                     /label=rbs
     RBS             994..1003
                     /label=BBa_J15001
     CDS             1010..1939
                     /label=crtB coding sequence
     CDS             1010..1942
                     /label=BBa_K118002
     misc_feature    1951..1953
                     /label=SacI
     RBS             1954..1960
                     /label=rbs
     RBS             1951..1960
                     /label=BBa_J15001
     misc_feature    2444..2449
                     /label=PstI site removed
     misc_feature    2855..2860
                     /label=PstI site removed
     CDS             1967..3445
                     /label=crtI coding sequence
     CDS             1967..3448
                     /label=BBa_K118003
     misc_feature    64..3448
                     /label=BBa_K274100
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gaggaggtac tagatgacgg tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc
      121 ggagcagtta ctggctgata ttgatcgacg ccttgatcag ttattgcccg tggagggaga
      181 acgggatgtt gtgggtgccg cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg
      241 ccccatgttg ctgttgctga ccgcccgcga tctgggttgc gctgtcagcc atgacggatt
      301 actggatttg gcctgtgcgg tggaaatggt ccacgcggct tcgctgatcc ttgacgatat
      361 gccctgcatg gacgatgcga agctgcggcg cggacgccct accattcatt ctcattacgg
      421 agagcatgtg gcaatactgg cggcggttgc cttgctgagt aaagcctttg gcgtaattgc
      481 cgatgcagat ggcctcacgc cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc
      541 catcggcatg caaggattgg ttcagggtca gttcaaggat ctgtctgaag gggataagcc
      601 gcgcagcgct gaagctattt tgatgacgaa tcactttaaa accagcacgc tgttttgtgc
      661 ctccatgcag atggcctcga ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca
      721 tcgtttttca cttgatcttg gtcaggcatt tcaactgctg gacgatttga ccgatggcat
      781 gaccgacacc ggtaaggata gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt
      841 aggcccgagg gcggttgaag aacgtctgag acaacatctt cagcttgcca gtgagcatct
      901 ctctgcggcc tgccaacacg ggcacgccac tcaacatttt attcaggcct ggtttgacaa
      961 aaaactcgct gccgtcagtt aataatacta gagctcaagg aggtactaga tgaataatcc
     1021 gtcgttactc aatcatgcgg tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc
     1081 ctcaaagtta tttgatgcaa aaacccggcg cagcgtactg atgctctacg cctggtgccg
     1141 ccattgtgac gatgttattg acgatcagac gctgggcttt caggcccggc agcctgcctt
     1201 acaaacgccc gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg cctatgcagg
     1261 atcgcagatg cacgaaccgg cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat
     1321 cgccccggct tacgcgtttg atcatctgga aggcttcgcc atggatgtac gcgaagcgca
     1381 atacagccaa ctggatgata cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt
     1441 gatgatggcg caaatcatgg gcgtgcggga taacgccacg ctggaccgcg cctgtgacct
     1501 tgggctggca tttcagttga ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg
     1561 ccgctgttat ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag agaattatgc
     1621 ggcacctgaa aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga
     1681 accttactat ttgtctgcca cagccggcct ggcagggttg cccctgcgtt ccgcctgggc
     1741 aatcgctacg gcgaagcagg tttaccggaa aataggtgtc aaagttgaac aggccggtca
     1801 gcaagcctgg gatcagcggc agtcaacgac cacgcccgaa aaattaacgc tgctgctggc
     1861 cgcctctggt caggccctta cttcccggat gcgggctcat cctccccgcc ctgcgcatct
     1921 ctggcagcgc ccgctctaat aatactagag ctcaaggagg tactagatga aaccaactac
     1981 ggtaattggt gcaggcttcg gtggcctggc actggcaatt cgtctacaag ctgcggggat
     2041 ccccgtctta ctgcttgaac aacgtgataa acccggcggt cgggcttatg tctacgagga
     2101 tcaggggttt acctttgatg caggcccgac ggttatcacc gatcccagtg ccattgaaga
     2161 actgtttgca ctggcaggaa aacagttaaa agagtatgtc gaactgctgc cggttacgcc
     2221 gttttaccgc ctgtgttggg agtcagggaa ggtctttaat tacgataacg atcaaacccg
     2281 gctcgaagcg cagattcagc agtttaatcc ccgcgatgtc gaaggttatc gtcagtttct
     2341 ggactattca cgcgcggtgt ttaaagaagg ctatctaaag ctcggtactg tccctttttt
     2401 atcgttcaga gacatgcttc gcgccgcacc tcaactggcg aaactgcaag catggagaag
     2461 cgtttacagt aaggttgcca gttacatcga agatgaacat ctgcgccagg cgttttcttt
     2521 ccactcgctg ttggtgggcg gcaatccctt cgccacctca tccatttata cgttgataca
     2581 cgcgctggag cgtgagtggg gcgtctggtt tccgcgtggc ggcaccggcg cattagttca
     2641 ggggatgata aagctgtttc aggatctggg tggcgaagtc gtgttaaacg ccagagtcag
     2701 ccatatggaa acgacaggaa acaagattga agccgtgcat ttagaggacg gtcgcaggtt
     2761 cctgacgcaa gccgtcgcgt caaatgcaga tgtggttcat acctatcgcg acctgttaag
     2821 ccagcaccct gccgcggtta agcagtccaa caaactgcaa actaagcgca tgagtaactc
     2881 tctgtttgtg ctctattttg gtttgaatca ccatcatgat cagctcgcgc atcacacggt
     2941 ttgtttcggc ccgcgttacc gcgagctgat tgacgaaatt tttaatcatg atggcctcgc
     3001 agaggacttc tcactttatc tgcacgcgcc ctgtgtcacg gattcgtcac tggcgcctga
     3061 aggttgcggc agttactatg tgttggcgcc ggtgccgcat ttaggcaccg cgaacctcga
     3121 ctggacggtt gaggggccaa aactacgcga ccgtattttt gcgtaccttg agcagcatta
     3181 catgcctggc ttacggagtc agctggtcac gcaccggatg tttacgccgt ttgattttcg
     3241 cgaccagctt aatgcctatc atggctcagc cttttctgtg gagcccgttc ttacccagag
     3301 cgcctggttt cggccgcata accgcgataa aaccattact aatctctacc tggtcggcgc
     3361 aggcacgcat cccggcgcag gcattcctgg cgtcatcggc tcggcaaaag cgacagcagg
     3421 tttgatgctg gaggatctga tataataa
//