GH371

BBa_V1011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_V1011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-06-28 11:00:00
Date modified: 2015-05-08 01:14:53

E. coli strain GH371
5-fluorouracil resistant upp mutant of GeneHogs



Types
DnaRegion

Roles
Cell

chromosome

Description

GeneHogs is a phage-resistant variant of DH10B (see [[www.invitrogen.com/content/sfs/manuals/genehogs_man.pdf]]. GH371 is an upp mutant of GeneHogs generated by lambda-red recombination.

GH371 is resistant to ~10 ug/mL 5-Fluorouracil and has been used for negative genetic selections (See Chin and coworkers: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abstract&list_uids=16408021&query_hl=5&itool=pubmed_docsum]

Notes

N/A

Source

Generated by lambda-red recombination of a PCR product amplified with oligonucleotides ca371F and ca371R from pKD13 in GeneHogs transformed with pKD46. See Datsenko&Wanner for more information on the methodology: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=Abstract&list_uids=10829079&query_hl=8&itool=pubmed_docsum]

igem#experience
Works
 
igem#sampleStatus
No part sequence
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_V1011/1