| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_Y00029_sequence (Version 1)
|
Description
Y00029 is S. pombe gene SPBC1921.07c
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=protein&cmd=search&term=NP_596000.2 recoded for expression in S. cerevisiae. The pombe gene is the homolog of the cerevisiae SGF29
http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf29, a subunit of the ATP-independent chromatin remodelling complex SAGA. Deletion of this gene in S. cerevisiae results in a viable strain. Requirements for SGF29 in SAGA function are largely unexplored. Unlike many of the SAGA subunits, the pombe and the cerevisiae gene encode proteins of abour the same size (259 aa in cerevisiae and 244 in pombe). The gene has been recoded for expression in S. cerevisiae by DNA 2.0, although it's not clear that this recoding will be necessary for expresssion. The recoded gene is 78% identical to the original pombe gene (Identities = 568/723).
Notes
insured no biobrick sites in ORF during optimization
Source
DNA 2.0 optimization of pombe genomic sequence
