BBa_Y00073
1
BBa_Y00073
S. pombe gene SPCC126.04c, coded for S. cerevisiae expression
2006-06-21T11:00:00Z
2015-05-08T01:14:54Z
S. pombe gene recoded for S. cerevisiae expression
SPCC126.04c is the pombe homolog of SGF73, a subunit of the SAGA ATP-independent chromatin remodelling complex in S. cerevisiae. Deletion of this subunit from S. cerevisiae leads to viable cells but poor expression of PAU genes under hypoxic conditions. Other phenotypes may also exist but are currently undescribed. The part BBa_Y00073 is the pombe SPCC126.04c ORF (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=68037958&itemID=1&view=gbwithparts) that has been codon optimized by DNA2.0 for cerevisiae expression. This may or may not be needed for optimal expression in cerevisiae, though the recoding does leave only 77% (794/1028) bp identities.
false
false
_43_
0
314
1
It's complicated
false
codon optimization for expression in new species
false
Natalie Kuldell
annotation1881735
1
suffix
range1881735
1
1066
1094
annotation1881734
1
prefix
range1881734
1
1
30
BBa_Y00073_sequence
1
gtttcttcgaattcgcggccgcttctagagatgctaaccgaacaacaaactttggaattatttcctgagaactgggaaagaaatgaacaggttttgttgaaatatccagatggtttaccaaaagatgtgttggcagatcaaagagttgacaataccgaatcacaggtgaaagatgaaatcacttctaatatcactaaccaaactcagatacttctgccagcagattcttttccgcagtatggctcatatccaaacaatagtgaattggattacgttgtttgtacgaagtgtgatagaccattcttgtccgaatatattgaagaccatcactcatcctgtaatggtatcaaacctcccaaacctcaatttgaaccggtagctaattcacaggtcttaaacaaagatgtcaataacggcaataatgctcccattaaaaacggagtcaaatcgacagcaaagggctctgctggaaatcatgagaaaaattctgtgaatggtcagaagaaccccgagatgccccctaaaaggcgtaaaactgaggagaacaagaaacccactaaatccgcgttgccaaagaaagaggcttcgaaaaagaagaatccaaaggtgaagggcccggttgacgtggagaaacagtgtggggtcttattaccaaatggccagatgtgtgctcgttctctaacgtgtaaaacacactcaatgtcaagtaaaagggcagttccgggacgttcacaaccctatgatgtgcttttggcggcatgtcaaaagaagaaccaggtcaagatccaaaggcaaatcttggaaactgccaaggagtccgaagacaatcaacatcaagctccggttgacagtgacgaagaggtgtcctttattatgaatgttttacaaaaatctggcaatcaaccattggaacaaaaagtcttcttgccagttaaacgtagacatagttattttaggactagagaattaattgcggcagctttccgtcatggtgagcaagggatgcaggtaactggtacgattctgggccgtgtgatacctttctcagcccgtcaacctctgtaatactagtagcggccgctgcaggaagaaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z