LOCUS pSB1A1 2708 bp DNA linear 06-May-2024 DEFINITION pUC19 with a BioBrick cloning site (Replaced by pSB1A3) ACCESSION pSB1A1 VERSION pSB1A1.1 FEATURES Location/Qualifiers primer_bind 70..86 /label=M13Reverse priming site misc_feature 112..112 /label=P(LAC) transcription start site promoter 119..124 /label=P(LAC) -10 site promoter 143..148 /label=P(LAC) -35 site misc_feature 168..180 /label=CAP protein binding site misc_feature 60..289 /label=Lac operon misc_feature 472..472 /label=ORI misc_feature 472..1024 /label=RNAII transcript misc_feature 472..1060 /label=origin of replication misc_feature 914..1021 /label=RNAI transcript promoter 878..883 /label=RNAI transcript promoter -35 site promoter 900..906 /label=RNAI transcript promoter -35 site promoter 1034..1039 /label=RNAII transcript promoter -10 site promoter 1055..1060 /label=RNAII transcript promoter -35 site misc_feature 290..2291 /label=pBR322 CDS 1231..2091 /label=beta-lactamase (bla; amp-r) CDS 2023..2091 /label=beta-lactamase signal peptide misc_feature 2126..2126 /label=bla RNA transcription start promoter 2135..2140 /label=P(BLA) -10 site promoter 2156..2161 /label=P(BLA) -35 site misc_feature 2292..2428 /label=pBR322 primer_bind 2670..2685 /label=M13 Forward priming site misc_feature 1..21 /label=BioBricks suffix misc_feature 2687..2708 /label=BioBricks prefix ORIGIN 1 tactagtagc ggccgctgca gggatcctct agagtcgacc tgcaggcatg caagcttggc 61 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 901 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 1021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 1081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 1201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 1261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 1321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 1381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 1441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 1501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 1561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 1621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 1681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 1741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 1801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 1861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 1921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 1981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 2041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 2101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 2161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 2221 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 2281 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 2341 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 2401 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 2461 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 2521 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 2581 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 2641 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgcggccgc 2701 ttctagag //