LOCUS       pSB1AC0                 3031 bp    DNA     linear       29-Apr-2024
DEFINITION  pSB1AC0
ACCESSION   pSB1AC0
VERSION     pSB1AC0.1  
FEATURES             Location/Qualifiers
     misc_feature    26..27
                     /label=2bp deletion
     stem_loop       22..56
                     /label=E. coli his operon terminator stem loop
     stem_loop       14..85
                     /label=E. coli his operon terminator
     primer_bind     149..168
                     /label=VR
     misc_feature    268..268
                     /label=ORI
     CDS             253..867
                     /label=rep (pMB1)
     stem_loop       1034..1139
                     /label=T0 terminator
     misc_feature    1229..1229
                     /label=Silent G->A mutation
     CDS             1155..1813
                     /label=CmR
     misc_feature    1911..1911
                     /label=C insertion
     CDS             1925..2785
                     /label=AmpR
     promoter        2820..2820
                     /label=P(BLA)
     primer_bind     2908..2927
                     /label=VF2
     stem_loop       2997..3016
                     /label=upstream terminator stem loop
     misc_feature    2984..2987
                     /label=end of upstream terminator
ORIGIN
        1 gcggccgctg cagtccggca aaaaaacggg caaggtgtca ccaccctgcc ctttttcttt
       61 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
      121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      241 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
      721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1021 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1081 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1141 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1201 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1261 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1321 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1381 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1441 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1501 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1561 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1621 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1681 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1741 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1801 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1861 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
     1921 cgagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1981 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2041 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2101 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2161 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2221 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2281 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2341 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2401 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2461 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2521 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2581 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2641 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2701 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2761 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2821 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2881 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2941 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     3001 tagctttcgc taaggatgat ttctggaatt c
//