Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-06 11:00:00
Date modified: 2015-05-08 01:14:55
High copy BioBrick assembly plasmid
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | pSB1AC3_sequence (Version 1) |
Description
pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.Source
pSB1A3 with added Cm resistance| Sequence Annotation | Location | Component / Role(s) |
| Suffix Prefix rep (pMB1) (rev) ORI Cm R (rev) APr (rev) terminator stem loop terminator stem loop VF2 VR T0 terminator Silent g->a mutation found during sequencing deletion of two bases E. coli his operon terminator end of terminator | 1,21 3034,3055 261,875 276,276 1163,1821 1933,2793 30,64 3005,3024 2916,2935 157,176 1042,1147 1237,1237 34,35 22,93 2992,2995 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/cds CDS sequence_feature feature/misc feature/cds CDS CDS feature/cds stem_loop feature/stem_loop feature/stem_loop stem_loop primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding stem_loop feature/stem_loop point_mutation feature/s_mutation feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |