pSB1AK3

pSB1AK3 Version 1

Component

Source:
http://parts.igem.org/Part:pSB1AK3
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Registry
Date created: 2004-07-27 11:00:00
Date modified: 2015-05-08 01:14:55

High copy BioBrick assembly plasmid



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences pSB1AK3_sequence (Version 1)

Description

pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Sequence Annotation Location Component / Role(s)
Suffix
Prefix
rep (pMB1) (rev)
kanR (rev)
ampR (rev)
ORI
terminator stem loop
terminator stem loop
VF2
VR
deletion of two bases
E. coli his terminator
end of terminator
1,21
3168,3189
261,875
1097,1912
2067,2927
276,276
3139,3158
30,64
3050,3069
157,176
34,35
22,93
3126,3129
feature/BioBrick engineered_region
feature/BioBrick engineered_region
CDS feature/cds
CDS feature/cds
feature/cds CDS
sequence_feature feature/misc
stem_loop feature/stem_loop
stem_loop feature/stem_loop
feature/primer_binding primer_binding_site
feature/primer_binding primer_binding_site
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
pSB1AK3/1