Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-05 11:00:00
Date modified: 2015-05-08 01:14:55
PSB1AK3 with ccdB insert
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | pSB1AK3_1_sequence (Version 1) |
Description
Target plasmid for three antibiotic assembly. The ccdB insert prevents growth of the unmodified plasmid in normal cloning strains, allowing selection of only correctly assembled recombinants.| Sequence Annotation | Location | Component / Role(s) |
| kanR (rev) ampR (rev) ccdB (rev) ccdA- (rev) rep (pMB1) (rev) BB-Pre BB-Post Vf Vr terminator terminator ORI ccD Operon (rev) VF2 | 1912,2727 2882,3742 169,474 476,696 1076,1690 120,135 818,836 3785,3804 972,991 90,109 845,879 1091,1091 140,816 1,20 | feature/cds CDS feature/cds CDS CDS feature/cds feature/cds CDS feature/misc sequence_feature feature/BioBrick engineered_region engineered_region feature/BioBrick primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/stem_loop stem_loop stem_loop feature/stem_loop feature/misc sequence_feature feature/misc sequence_feature feature/primer_binding primer_binding_site |