Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-06 11:00:00
Date modified: 2015-05-08 01:14:55
High copy BioBrick assembly plasmid
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | pSB1AT3_sequence (Version 1) |
Description
pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).Source
pSB1A3 with added Tet resistance| Sequence Annotation | Location | Component / Role(s) |
| Suffix Prefix rep (pMB1) (rev) Tet-r APr (rev) ORI VR VF2 terminator stem loop terminator stem loop deletion of two bases E. coli his operon terminator end of terminator | 1,21 3425,3446 261,875 1127,2317 2324,3184 276,276 157,176 3307,3326 3396,3415 30,64 34,35 22,93 3383,3386 | feature/BioBrick engineered_region feature/BioBrick engineered_region feature/cds CDS CDS feature/cds CDS feature/cds sequence_feature feature/misc feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site stem_loop feature/stem_loop feature/stem_loop stem_loop feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature |