Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Che
Date created: 2008-09-07 11:00:00
Date modified: 2015-05-08 01:14:55
High copy BioBrick assembly plasmid
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | pSB1K3_sequence (Version 1) |
Description
This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.Notes
Eliminated ampicillin resistance.Source
This plasmid was derived from pSB1AK3-P1010.| Sequence Annotation | Location | Component / Role(s) |
| BioBrick suffix E. coli his operon terminator Stem loop Verification reverse (VR) primer binding site rep (pMB1) ORI Kanamycin resistance marker G->C mutation to remove XhoI site repeat region Verification forward (VF2) primer binding site end of terminator Stem loop BioBrick prefix | 1,21 22,93 30,64 157,176 261,875 276,276 1097,1912 1880,1880 2017,2060 2067,2086 2143,2146 2156,2175 2185,2206 | feature/dna sequence_feature stem_loop feature/stem_loop stem_loop feature/stem_loop primer_binding_site feature/primer_binding feature/misc sequence_feature feature/misc sequence_feature CDS feature/cds point_mutation feature/s_mutation sequence_feature feature/misc feature/primer_binding primer_binding_site stem_loop feature/stem_loop stem_loop feature/stem_loop feature/dna sequence_feature |