Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Che
Date created: 2008-09-07 11:00:00
Date modified: 2015-05-08 01:14:55
High copy BioBrick assembly plasmid
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | pSB1T3_sequence (Version 1) |
Description
This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.Notes
The ampicillin resistance marker needed to be eliminated.Source
Austin Che constructed this plamid from pSB1AT3-P1010.| Sequence Annotation | Location | Component / Role(s) |
| two base pair AC deletion BioBrick suffix Stem loop E. coli his operon terminator Verification reverse (VR) primer binding site ORI rep (pMB1) Promoter P1 Bind Site 1 Bind Site 2 Bind Site 3 Promoter P2 Bind Site 4 Bind Site 5 Tetracycline resistance C->T mutation Verifiction forward (VF2) primer binding site end of terminator Stem loop BioBrick prefix | 34,35 1,21 30,64 22,93 157,176 276,276 261,875 1068,1074 1065,1068 1080,1083 1094,1097 1084,1090 1108,1111 1121,1124 1127,2317 2175,2175 2324,2343 2400,2403 2413,2432 2442,2463 | feature/mutation sequence_alteration feature/dna sequence_feature stem_loop feature/stem_loop stem_loop feature/stem_loop feature/primer_binding primer_binding_site feature/misc sequence_feature feature/misc sequence_feature feature/promoter promoter feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site promoter feature/promoter feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/cds CDS sequence_alteration feature/mutation feature/primer_binding primer_binding_site stem_loop feature/stem_loop stem_loop feature/stem_loop feature/dna sequence_feature |