LOCUS       pSB1T3                  2461 bp    DNA     linear       03-May-2024
DEFINITION  High copy BioBrick assembly plasmid
ACCESSION   pSB1T3
VERSION     pSB1T3.1  
FEATURES             Location/Qualifiers
     misc_feature    34..35
                     /label=two base pair AC deletion
     misc_feature    1..21
                     /label=BioBrick suffix
     stem_loop       30..64
                     /label=Stem loop
     stem_loop       22..93
                     /label=E. coli his operon terminator
     primer_bind     157..176
                     /label=Verification reverse (VR) primer binding site
     misc_feature    276..276
                     /label=ORI
     misc_feature    261..875
                     /label=rep (pMB1)
     promoter        1068..1074
                     /label=Promoter P1
     misc_feature    1065..1068
                     /label=Bind Site 1
     misc_feature    1080..1083
                     /label=Bind Site 2
     misc_feature    1094..1097
                     /label=Bind Site 3
     promoter        1084..1090
                     /label=Promoter P2
     misc_feature    1108..1111
                     /label=Bind Site 4
     misc_feature    1121..1124
                     /label=Bind Site 5
     CDS             1127..2317
                     /label=Tetracycline resistance
     misc_feature    2175..2175
                     /label=C->T mutation
     primer_bind     2324..2343
                     /label=Verifiction forward (VF2) primer binding site
     stem_loop       2400..2403
                     /label=end of terminator
     stem_loop       2413..2432
                     /label=Stem loop
     misc_feature    2442..2463
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
       61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1021 ttggtctgac agctcgagat tctcatgttt gacagcttat catcgataag ctttaatgcg
     1081 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     1141 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     1201 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     1261 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
     1321 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     1381 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     1441 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1501 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1561 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1621 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1681 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1741 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     1801 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     1861 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     1921 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     1981 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     2041 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     2101 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     2161 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     2221 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     2281 gtcgcggtgc atggagccgg gccacctcga cctaactcga gtgccacctg acgtctaaga
     2341 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     2401 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
     2461 g
//