Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2004-06-28 11:00:00
Date modified: 2015-05-08 01:14:55
Low to medium copy BioBrick standard vector
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | pSB3K3_sequence (Version 1) |
Description
pSB3K3-1 is a medium copy plasmid with kanamycin resistance. It has a p15A pMR101-derived replication origin, copy number 20-30. (Lutz and Bujard, 1997)pSB3K3-1 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into the vectorl. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
| Sequence Annotation | Location | Component / Role(s) |
| Prefix Suffix kanR (rev) p15A ORI (rev) VF2 VR deletion of two bases E. coli his operon terminator terminator stem loop terminator stem loop end of terminator | 2729,2750 1,21 239,1054 1386,2210 2611,2630 157,176 34,35 22,93 30,64 2700,2719 2687,2690 | feature/BioBrick engineered_region engineered_region feature/BioBrick CDS feature/cds CDS feature/cds primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/misc sequence_feature feature/misc sequence_feature feature/stem_loop stem_loop stem_loop feature/stem_loop sequence_feature feature/misc |