LOCUS       pSB4C1                  3155 bp    DNA     linear       23-May-2024
DEFINITION  pCK01 converted into BioBrick vector (formerly BBa_I739202)
ACCESSION   pSB4C1
VERSION     pSB4C1.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     CDS             490..1440
                     /label=pSC101 replication initiator protein
     CDS             2311..2970
                     /label=chloramphenicol acetyltransferase
     misc_feature    1508..1508
                     /label=C->T mutation to eliminate SpeI site
     primer_bind     1491..1529
                     /label=Mutagenesis primer
     misc_feature    3134..3155
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca gatatgaatc ggccaacgcg cggggagagg cggtttgcgt
       61 attgggcgct atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
      121 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
      181 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
      241 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
      301 atctgttcat ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct
      361 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg
      421 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
      481 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
      541 ttgcgtgagc catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa
      601 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
      661 tagtccgtta cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
      721 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
      781 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
      841 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
      901 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
      961 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     1021 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     1081 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     1141 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     1201 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     1261 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     1321 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     1381 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     1441 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     1501 ataattatta gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     1561 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     1621 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     1681 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     1741 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     1801 aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca
     1861 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     1921 tgaatggggg taaatggcac tacaggcgct gatgtccggc ggtgcttttg ccgttacgca
     1981 ccaccccgtc agtagctgaa caggagggac agctgataga aacagaagcc actggagcac
     2041 ctcaaaaaca ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc
     2101 gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
     2161 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
     2221 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     2281 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     2341 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     2401 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     2461 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     2521 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     2581 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
     2641 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
     2701 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
     2761 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
     2821 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     2881 gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     2941 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa
     3001 acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag
     3061 caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg
     3121 ctggtttacc ggtgaattcg cggccgcttc tagag
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