LOCUS pSB4T5 3735 bp DNA linear 08-May-2024 DEFINITION Low copy BioBrick standard vector ACCESSION pSB4T5 VERSION pSB4T5.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..183 /label=BBa_G0003 stem_loop 229..258 /label=Terminator misc_feature 320..687 /label=par region misc_feature 322..335 /label=inverted repeat misc_feature 341..345 /label=Tn3 integration site misc_feature 348..364 /label=inverted repeat misc_feature 375..398 /label=inverted repeat misc_feature 404..421 /label=inverted repeat misc_feature 818..900 /label=AT rich region misc_feature 899..918 /label=direct repeat misc_feature 920..939 /label=direct repeat misc_feature 952..971 /label=direct repeat misc_feature 997..1002 /label=mutated SpeI site promoter 1011..1016 /label=-35 misc_feature 1021..1042 /label=inverted repeat promoter 1029..1048 /label=promoter region promoter 1033..1038 /label=-10 misc_feature 1045..1067 /label=inverted repeat RBS 1053..1065 /label=RBS RBS 1062..1067 /label=RBS CDS 1069..2019 /label=repA misc_feature 1838..1838 /label=G to A difference with canonical pSC101 vector sequence misc_feature 1933..1933 /label=C to T difference with canonical pSC101 vector sequence misc_feature 2030..2044 /label=inverted repeat misc_feature 2095..2095 /label=G to C difference with canonical pSC101 vector sequence polyA_site 2130..2145 /label=putative polyA misc_feature 2146..2162 /label=inverted repeat misc_feature 2185..2185 /label=T to C difference with canonical pSC101 vector sequence misc_feature 184..2220 /label=BBa_I50042 misc_feature 2221..2228 /label=BBa_G0002 promoter 2229..2317 /label=tetR promoter CDS 2318..3511 /label=tetA misc_feature 2229..3511 /label=BBa_P1005 misc_feature 3512..3518 /label=BBa_G0004 stem_loop 3527..3561 /label=stem_loop terminator 3519..3590 /label=BBa_B0053 primer_bind 3591..3610 /label=VF2 primer 3591..3610 /label=BBa_G00100 stem_loop 3646..3667 /label=stem loop from bacteriophage T3 misc_feature 3638..3676 /label=identical to bacteriophage T3 terminator 3611..3688 /label=BBa_B0055 misc_feature 3690..3692 /label=stop codon (+2 frame) misc_feature 3689..3691 /label=stop codon (-1 frame) misc_feature 3694..3696 /label=stop codon (+3 frame) misc_feature 3698..3700 /label=stop codon (+1 frame) misc_feature 3697..3699 /label=stop codon (-2 frame) misc_feature 3693..3695 /label=stop codon (-3 frame) misc_feature 3689..3700 /label=BBa_B0042 misc_feature 3701..3705 /label=Topoisomerase I recognition site misc_feature 3709..3713 /label=Nt.BstNB I recognition site misc_feature 3706..3708 /label=Arbitrary sequence (could be any bases) misc_feature 3705..3705 /label=Generated T overhang misc_feature 3701..3713 /label=BBa_B0043 misc_feature 3714..3719 /label=EcoRI site misc_feature 3729..3734 /label=XbaI site misc_feature 3714..3735 /label=Prefix misc_feature 3720..3727 /label=NotI site misc_feature 3714..3735 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gagctgtcag accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac 241 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 301 tgagaatcca agcactaggg acagtaagac gggtaagcct gttgatgata ccgctgcctt 361 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg 421 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac 481 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc 541 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg 601 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag 661 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg 721 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc 781 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt 841 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa 901 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag 961 cagaatttac agatacccac aactcaaagg aaaaggacat gtaattatca ttgactagcc 1021 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta 1081 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc 1141 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa 1201 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa 1261 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc 1321 aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca 1381 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa 1441 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg 1501 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag 1561 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa 1621 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg 1681 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta 1741 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac 1801 aaaataccaa caaccattac atcagattcc tacctacata acggactaag aaaaacacta 1861 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac 1921 atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc 1981 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc 2041 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc 2101 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga 2161 gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac 2221 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 2281 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 2341 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 2401 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 2461 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 2521 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 2581 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 2641 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 2701 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 2761 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 2821 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 2881 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 2941 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 3001 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 3061 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 3121 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 3181 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 3241 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 3301 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 3361 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 3421 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 3481 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 3541 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 3601 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 3661 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 3721 cggccgcttc tagag //