LOCUS pSB5A5 7012 bp DNA linear 03-May-2024 DEFINITION pSB5A5 ACCESSION pSB5A5 VERSION pSB5A5.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 30..34 /label=Nt.BstNB I recognition site misc_feature 35..37 /label=Arbitrary sequence (could be any bases) misc_feature 38..42 /label=Topoisomerase I recognition site misc_feature 38..38 /label=Generated T overhang misc_feature 30..42 /label=BBa_B0044 misc_feature 52..54 /label=stop codon (+2 frame) misc_feature 51..53 /label=stop codon (-1 frame) misc_feature 56..58 /label=stop codon (+3 frame) misc_feature 60..62 /label=stop codon (+1 frame) misc_feature 59..61 /label=stop codon (-2 frame) misc_feature 55..57 /label=stop codon (-3 frame) misc_feature 51..62 /label=BBa_B0042 stem_loop 81..112 /label=stem loop misc_feature 74..113 /label=identical to E. coli MG1655 between tonB and yciA terminator 71..139 /label=BBa_B0054 primer_bind 148..167 /label=VR misc_feature 148..167 /label=BBa_G00102 stem_loop 189..208 /label=stem_loop terminator 176..216 /label=BBa_B0062 CDS 331..993 /label=cmR misc_feature 225..993 /label=BBa_P1004 misc_feature 1002..1704 /label=sopC (IncD) repeat region misc_feature 1931..1931 /label=A to T mutation (eliminates SpeI site) CDS 1778..2749 /label=parB misc_feature 3089..3089 /label=C to T mutation (eliminates PstI site) CDS 2749..3924 /label=parA misc_feature 1002..3922 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 misc_feature 4252..5641 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 misc_feature 4629..4629 /label=C to T mutation (eliminates PstI site) CDS 4503..5258 /label=repE misc_feature 5355..5374 /label=iteron (repeat region) misc_feature 5377..5396 /label=iteron (repeat region) misc_feature 5410..5429 /label=iteron (repeat region) misc_feature 5431..5450 /label=iteron (repeat region) misc_feature 5459..5459 /label=C to G mutation (eliminates XbaI site, noncoding region) misc_feature 5352..5641 /label=ori2 (oriS) single copy replication origin polyA_site 5495..5541 /label=A/T region misc_feature 5545..5553 /label=DnaA box misc_feature 5554..5561 /label=DnaA box misc_feature 1002..5641 /label=BBa_I50001 stem_loop 5658..5692 /label=stem_loop terminator 5650..5721 /label=BBa_B0053 primer_bind 5730..5749 /label=VF2 primer 5730..5749 /label=BBa_G00100 stem_loop 5793..5814 /label=stem loop from bacteriophage T3 misc_feature 5785..5823 /label=identical to bacteriophage T3 terminator 5758..5835 /label=BBa_B0055 misc_feature 5845..5847 /label=stop codon (+2 frame) misc_feature 5844..5846 /label=stop codon (-1 frame) misc_feature 5849..5851 /label=stop codon (+3 frame) misc_feature 5853..5855 /label=stop codon (+1 frame) misc_feature 5852..5854 /label=stop codon (-2 frame) misc_feature 5848..5850 /label=stop codon (-3 frame) misc_feature 5844..5855 /label=BBa_B0042 misc_feature 5864..5868 /label=Topoisomerase I recognition site misc_feature 5872..5876 /label=Nt.BstNB I recognition site misc_feature 5869..5871 /label=Arbitrary sequence (could be any bases) misc_feature 5868..5868 /label=Generated T overhang misc_feature 5864..5876 /label=BBa_B0043 misc_feature 5885..5890 /label=EcoRI site misc_feature 5900..5905 /label=XbaI site misc_feature 5885..5906 /label=Prefix misc_feature 5891..5898 /label=NotI site misc_feature 5885..5906 /label=BBa_G00000 promoter 5955..5960 /label=-35 promoter 5978..5983 /label=-10 CDS 6022..6330 /label=ccdB misc_feature 5915..6330 /label=BBa_P1011 misc_feature 6354..6968 /label=rep (pMB1) misc_feature 6953..6953 /label=ORI misc_feature 6365..6953 /label=origin of replication misc_feature 6401..6953 /label=RNAII transcript promoter 6542..6547 /label=RNAI transcript promoter -35 promoter 6520..6525 /label=RNAI transcript promoter -10 misc_feature 6404..6511 /label=RNAI transcript promoter 6386..6391 /label=RNAII transcript promoter -10 sequence promoter 6365..6370 /label=RNAII transcript promoter -35 sequence misc_feature 6599..6599 /label=G to A, nonfunctional mutation misc_feature 6603..6603 /label=G to A, nonfunctional mutation misc_feature 6612..6612 /label=G to A, nonfunctional mutation misc_feature 6641..6641 /label=C to T, nonfunctional mutation misc_feature 6805..6805 /label=C to T, nonfunctional mutation misc_feature 6339..7012 /label=BBa_I50020 misc_feature 5915..7012 /label=BBa_I52000 ORIGIN 1 tactagtagc ggccgctgca gtactagagg agtcactaag ggtactagag ttagttagtt 61 agtactagag attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc 121 ccttggggtt atcattgggt actagaggct cactcaaagg cggtaattac tagagcagat 181 aaaaaaaatc cttagctttc gctaaggatg atttcttact agaggttgat cgggcacgta 241 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta 301 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcacggg atataccacc 361 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 421 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 481 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaacgctcac 541 ccggagtttc gtatggccat gaaagacggt gagctggtga tctgggatag tgttcaccct 601 tgttacaccg ttttccatga gcaaactgaa acgttttcgt ccctctggag tgaataccac 661 gacgatttcc ggcagtttct ccacatatat tcgcaagatg tggcgtgtta cggtgaaaac 721 ctggcctatt tccctaaagg gtttattgag aatatgtttt ttgtctcagc caatccctgg 781 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 841 ttcacgatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgatccag 901 gttcatcatg ccgtttgtga tggcttccat gtcggccgca tgcttaatga attacaacag 961 tactgtgatg agtggcaggg cggggcgtaa taatactaga gacttgcatc ggatgcagcc 1021 cggtgaacgt gccggcacgg cctgggtaac caggtatttt gtccacataa ccgtgcgcaa 1081 aatgttgtgg ataagcagga cacagcagca atccacagca ggcatacaac cgcacaccga 1141 ggttactccg ttctacaggt tacgacgaca tgtcaatact tgcccttgac aggcattgat 1201 ggaatcgtag tctcacgctg atagtctgat cgacaataca agtgggaccg tggtcccaga 1261 ccgataatca gaccgacaac acgagtggga tcgtggtccc agactaataa tcagaccgac 1321 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt 1381 ggttccagac taataatcag accgacgata cgagtgggac cgtggtccca gactaataat 1441 cagaccgacg atacgagtgg gaccatggtc ccagactaat aatcagaccg acgatacgag 1501 tgggaccgtg gtcccagtct gattatcaga ccgacgatac gagtgggacc gtggtcccag 1561 actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga 1621 cgatacgagt gggaccgtgg tcccagtctg attatcagac cgacgataca agtggaacag 1681 tgggcccaga gagaatattc aggccagtta tgctttctgg cctgtaacaa aggacattaa 1741 gtaaagacag ataaacgtag actaaaacgt ggtcgcatca gggtgctggc ttttcaagtt 1801 ccttaagaat ggcctcaatt ttctctatac actcagttgg aacacgagac ctgtccaggt 1861 taagcaccat tttatcgccc ttatacaata ctgtcgctcc aggagcaaac tgatgtcgtg 1921 agcttaaact tgttcttgat gcagatgacg ttttaagcac agaagttaaa agagtgataa 1981 cttcttcagc ttcaaatatc accccagctt ttttctgctc atgaaggtta gatgcctgct 2041 gcttaagtaa ttcctcttta tctgtaaagg ctttttgaag tgcatcacct gaccgggcag 2101 atagttcacc ggggtgagaa aaaagagcaa caactgattt aggcaatttg gcggtgttga 2161 tacagcgggt aataatctta cgtgaaatat tttccgcatc agccagcgca gaaatatttc 2221 cagcaaattc attctgcaat cggcttgcat aacgctgacc acgttcataa gcacttgttg 2281 ggcgataatc gttacccaat ctggataatg cagccatctg ctcatcatcc agctcgccaa 2341 ccagaacacg ataatcactt tcggtaagtg cagcagcttt acgacggcga ctcccatcgg 2401 caatttctat gacaccagat actcttcgac cgaacgccgg tgtctgttga ccagtcagta 2461 gaaaagaagg gatgagatca tccagtgcgt cctcagtaag cagctcctgg tcacgttcat 2521 tacctgacca tacccgagag gtcttctcaa cactatcacc ccggagcact tcaagagtaa 2581 acttcacatc ccgaccacat acaggcaaag taatggcatt accgcgagcc attactccta 2641 cgcgcgcaat taacgaatcc accatcgggg cagctggtgt cgataacgaa gtatcttcaa 2701 ccggttgagt attgagcgta tgttttggaa taacaggcgc acgcttcatt atctaatctc 2761 ccagcgtggt ttaatcagac gatcgaaaat ttcattgcag acaggttccc aaatagaaag 2821 agcatttctc caggcaccag ttgaagagcg ttgatcaatg gcctgttcaa aaacagttct 2881 catccggatc tgacctttac caacttcatc cgtttcacgt acaacatttt ttagaaccat 2941 gcttccccag gcatcccgaa tttgctcctc catccacggg gactgagagc cattactatt 3001 gctgtatttg gtaagcaaaa tacgtacatc aggctcgaac cctttaagat caacgttctt 3061 gagcagatca cgaagcatat cgaaaaattg cagtgcggag gtgtagtcaa acaactcagc 3121 aggcgtggga acaatcagca catcagcagc acatacgaca ttaatcgtgc cgatacccag 3181 gttaggcgcg ctgtcaataa ctatgacatc atagtcatga gcaacagttt caatggccag 3241 tcggagcatc aggtgtggat cggtgggcag tttaccttca tcaaatttgc ccattaactc 3301 agtttcaata cggtgcagag ccagacagga aggaataatg tcaagccccg gccagcaagt 3361 gggctttatt gcataagtga catcgtcctt ttccccaaga tagaaaggca ggagagtgtc 3421 ttctgcatga atatgaagat ctggtaccca tccgtgatac attgaggctg ttccctgggg 3481 gtcgttacct tccacgagca aaacacgtag ccccttcaga gccagatcct gagcaagatg 3541 aacagaaact gaggttttgt aaacgccacc tttatgggca gcaaccccga tcaccggtgg 3601 aaatacgtct tcagcacgtc gcaatcgcgt accaaacaca tcacgcatat gattaatttg 3661 ttcaattgta taaccaacac gttgctcaac ccgtcctcga atttccatat ccgggtgcgg 3721 tagtcgccct gctttctcgg catctctgat agcctgagaa gaaaccccaa ctaaatccgc 3781 tgcttcacct attctccagc gccgggttat tttcctcgct tccgggctgt catcattaaa 3841 ctgtgcaatg gcgatagcct tcgtcatttc atgaccagcg tttatgcact ggttaagtgt 3901 ttccatgagt ttcattctga acatccttta atcattgctt tgcgtttttt tattaaatct 3961 tgcaatttac tgcaaagcaa caacaaaatc gcaaagtcat caaaaaaccg caaagttgtt 4021 taaaataaga gcaacactac aaaaggagat aagaagagca catacctcag tcacttatta 4081 tcactagcgc tcgccgcagc cgtgtaaccg agcatagcga gcgaactggc gaggaagcaa 4141 agaagaactg ttctgtcaga tagctcttac gctcagcgca agaagaaata tccaccgtgg 4201 gaaaaactcc aggtagaggt acacacgcgg atagccaatt cagagtaata aactgtgata 4261 atcaaccctc atcaatgatg acgaactaac ccccgatatc aagtcacatg acgaagggaa 4321 agagaaggaa atcaactgtg acaaactgcc ctcaaatttg gcttccttaa aaattacagt 4381 tcaaaaagta tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt gacaaattac 4441 cctcagtagg tcagaacaaa tgtgacgaac caccctcaaa tctgtgacag ataaccctca 4501 gactatcctg tcgtcatgga agtgatatcg cggaaggaaa atacgatatg agtcgtctgg 4561 cggcctttct ttttctcaat gtatgagagg cgcattggag ttctgctgtt gatctcatta 4621 acacagactt gcaggaagcg gcggcggaag tcaggcatac gctggtaact ttgaggcagc 4681 tggtaacgct ctatgatcca gtcgattttc agagagacga tgcctgagcc atccggctta 4741 cgatactgac acagggattc gtataaacgc atggcatacg gattggtgat ttcttttgtt 4801 tcactaagcc gaaactgcgt aaaccggttc tgtaacccga taaagaaggg aatgagatat 4861 gggttgatat gtacactgta aagccctctg gatggactgt gcgcacgttt gataaaccaa 4921 ggaaaagatt catagccttt ttcatcgccg gcatcctctt cagggcgata aaaaaccact 4981 tccttccccg cgaaactctt caatgcctgc cgtatatcct tactggcttc cgcagaggtc 5041 aatccgaata tttcagcata tttagcaaca tggatctcgc agataccgtc atgttcctgt 5101 agggtgccat cagattttct gatctggtca acgaacagat acagcatacg tttttgatcc 5161 cgggagagac tatatgccgc ctcagtgagg tcgtttgact ggacgattcg cgggctattt 5221 ttacgtttct tgtgattgat aaccgctgtt tccgccatga cagatccatg tgaagtgtga 5281 caagttttta gattgtcaca ctaaataaaa aagagtcaat aagcagggat aactttgtga 5341 aaaaacagct tcttctgagg gcaatttgtc acagggttaa gggcaatttg tcacagacag 5401 gactgtcatt tgagggtgat ttgtcacact gaaagggcaa tttgtcacaa caccttctgt 5461 agaaccagca tggataaagg cctacaaggc gctctaaaaa agaagatcta aaaactataa 5521 aaaaaataat tataaaaata tccccgtgga taagtggata accccaaggg aagttttttc 5581 aggcatcgtg tgtaagcaga atatataagt gctgttccct ggtgcttcct cgctcactcg 5641 atactagagt ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa 5701 ccgaaaagat tacttcgcgt ttactagagt gccacctgac gtctaagaat actagagaag 5761 gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg agccagtgag 5821 ttgattgcta cgtaatacta gagttagtta gttagtacta gagcccttag tgactctact 5881 agaggaattc gcggccgctt ctagagtact agagggctta ctaaaagcca gataacagta 5941 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag 6001 tatgtcaaaa agaggtatgc tatgcagttt aaggtttaca cctataaacg tgagagccgt 6061 tatcgtctgt ttgtggacgt acagagtgat attattgaca cgcctgggcg tcgcatggtg 6121 attcccctgg ccagcgcacg tctgctttca gataaagtct cccgtgaact ttacccggtg 6181 gtgcatatcg gggacgaaag ctggcgcatg atgaccaccg atatggccag cgtgccggtt 6241 tccgtgatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc 6301 attaacctga tgttctgggg aatataataa tactagagcc cgtagaaaag atcaaaagat 6361 cttcttgaga tccttttttt ctgcgcgtaa tctgctactt gcaaacaaaa aaaccaccgc 6421 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 6481 gctttagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 6541 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtag 6601 ctactgccag tagcgataag tcgtgtctta ccgggttgga ttcaagacga tagttaccgg 6661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 6721 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 6781 aagggagaaa ggcggacagg tatctggtaa gcggcagggt cggaacagga gagcgcacga 6841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 6901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 6961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac at //