LOCUS pSB5AC5 7858 bp DNA linear 04-May-2024 DEFINITION pSB5AC5-P1011.I50020 ACCESSION pSB5AC5 VERSION pSB5AC5.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 30..34 /label=Nt.BstNB I recognition site misc_feature 35..37 /label=Arbitrary sequence (could be any bases) misc_feature 38..42 /label=Topoisomerase I recognition site misc_feature 38..38 /label=Generated T overhang misc_feature 30..42 /label=BBa_B0044 misc_feature 52..54 /label=stop codon (+2 frame) misc_feature 51..53 /label=stop codon (-1 frame) misc_feature 56..58 /label=stop codon (+3 frame) misc_feature 60..62 /label=stop codon (+1 frame) misc_feature 59..61 /label=stop codon (-2 frame) misc_feature 55..57 /label=stop codon (-3 frame) misc_feature 51..62 /label=BBa_B0042 stem_loop 81..112 /label=stem loop misc_feature 74..113 /label=identical to E. coli MG1655 between tonB and yciA terminator 71..139 /label=BBa_B0054 primer_bind 148..167 /label=VR misc_feature 148..167 /label=BBa_G00102 stem_loop 189..208 /label=stem_loop terminator 176..216 /label=BBa_B0062 CDS 248..907 /label=CmR misc_feature 225..1013 /label=BBa_P1009 stem_loop 1035..1054 /label=stem_loop terminator 1022..1062 /label=BBa_B0062 CDS 1177..1839 /label=cmR misc_feature 1071..1839 /label=BBa_P1004 misc_feature 1848..2550 /label=sopC (IncD) repeat region misc_feature 2777..2777 /label=A to T mutation (eliminates SpeI site) CDS 2624..3595 /label=parB misc_feature 3935..3935 /label=C to T mutation (eliminates PstI site) CDS 3595..4770 /label=parA misc_feature 1848..4768 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 misc_feature 5098..6487 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 misc_feature 5475..5475 /label=C to T mutation (eliminates PstI site) CDS 5349..6104 /label=repE misc_feature 6201..6220 /label=iteron (repeat region) misc_feature 6223..6242 /label=iteron (repeat region) misc_feature 6256..6275 /label=iteron (repeat region) misc_feature 6277..6296 /label=iteron (repeat region) misc_feature 6305..6305 /label=C to G mutation (eliminates XbaI site, noncoding region) misc_feature 6198..6487 /label=ori2 (oriS) single copy replication origin polyA_site 6341..6387 /label=A/T region misc_feature 6391..6399 /label=DnaA box misc_feature 6400..6407 /label=DnaA box misc_feature 1848..6487 /label=BBa_I50001 stem_loop 6504..6538 /label=stem_loop terminator 6496..6567 /label=BBa_B0053 primer_bind 6576..6595 /label=VF2 primer 6576..6595 /label=BBa_G00100 stem_loop 6639..6660 /label=stem loop from bacteriophage T3 misc_feature 6631..6669 /label=identical to bacteriophage T3 terminator 6604..6681 /label=BBa_B0055 misc_feature 6691..6693 /label=stop codon (+2 frame) misc_feature 6690..6692 /label=stop codon (-1 frame) misc_feature 6695..6697 /label=stop codon (+3 frame) misc_feature 6699..6701 /label=stop codon (+1 frame) misc_feature 6698..6700 /label=stop codon (-2 frame) misc_feature 6694..6696 /label=stop codon (-3 frame) misc_feature 6690..6701 /label=BBa_B0042 misc_feature 6710..6714 /label=Topoisomerase I recognition site misc_feature 6718..6722 /label=Nt.BstNB I recognition site misc_feature 6715..6717 /label=Arbitrary sequence (could be any bases) misc_feature 6714..6714 /label=Generated T overhang misc_feature 6710..6722 /label=BBa_B0043 misc_feature 6731..6736 /label=EcoRI site misc_feature 6746..6751 /label=XbaI site misc_feature 6731..6752 /label=Prefix misc_feature 6737..6744 /label=NotI site misc_feature 6731..6752 /label=BBa_G00000 promoter 6801..6806 /label=-35 promoter 6824..6829 /label=-10 CDS 6868..7176 /label=ccdB misc_feature 6761..7176 /label=BBa_P1011 misc_feature 7200..7814 /label=rep (pMB1) misc_feature 7799..7799 /label=ORI misc_feature 7211..7799 /label=origin of replication misc_feature 7247..7799 /label=RNAII transcript promoter 7388..7393 /label=RNAI transcript promoter -35 promoter 7366..7371 /label=RNAI transcript promoter -10 misc_feature 7250..7357 /label=RNAI transcript promoter 7232..7237 /label=RNAII transcript promoter -10 sequence promoter 7211..7216 /label=RNAII transcript promoter -35 sequence misc_feature 7445..7445 /label=G to A, nonfunctional mutation misc_feature 7449..7449 /label=G to A, nonfunctional mutation misc_feature 7458..7458 /label=G to A, nonfunctional mutation misc_feature 7487..7487 /label=C to T, nonfunctional mutation misc_feature 7651..7651 /label=C to T, nonfunctional mutation misc_feature 7185..7858 /label=BBa_I50020 misc_feature 6761..7858 /label=BBa_I52000 ORIGIN 1 tactagtagc ggccgctgca gtactagagg agtcactaag ggtactagag ttagttagtt 61 agtactagag attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc 121 ccttggggtt atcattgggt actagaggct cactcaaagg cggtaattac tagagcagat 181 aaaaaaaatc cttagctttc gctaaggatg atttcttact agaggtccaa gcgagctcga 241 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc 301 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca 361 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca 421 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa 481 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat 541 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg 601 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca 661 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa 721 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg 781 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct 841 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt 901 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta 961 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactactaga 1021 gcagataaaa aaaatcctta gctttcgcta aggatgattt cttactagag gttgatcggg 1081 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt 1141 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cacgggatat 1201 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 1261 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 1321 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaac 1381 gctcacccgg agtttcgtat ggccatgaaa gacggtgagc tggtgatctg ggatagtgtt 1441 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcgtccct ctggagtgaa 1501 taccacgacg atttccggca gtttctccac atatattcgc aagatgtggc gtgttacggt 1561 gaaaacctgg cctatttccc taaagggttt attgagaata tgttttttgt ctcagccaat 1621 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 1681 cccgttttca cgatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 1741 atccaggttc atcatgccgt ttgtgatggc ttccatgtcg gccgcatgct taatgaatta 1801 caacagtact gtgatgagtg gcagggcggg gcgtaataat actagagact tgcatcggat 1861 gcagcccggt gaacgtgccg gcacggcctg ggtaaccagg tattttgtcc acataaccgt 1921 gcgcaaaatg ttgtggataa gcaggacaca gcagcaatcc acagcaggca tacaaccgca 1981 caccgaggtt actccgttct acaggttacg acgacatgtc aatacttgcc cttgacaggc 2041 attgatggaa tcgtagtctc acgctgatag tctgatcgac aatacaagtg ggaccgtggt 2101 cccagaccga taatcagacc gacaacacga gtgggatcgt ggtcccagac taataatcag 2161 accgacgata cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg 2221 gaccgtggtt ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact 2281 aataatcaga ccgacgatac gagtgggacc atggtcccag actaataatc agaccgacga 2341 tacgagtggg accgtggtcc cagtctgatt atcagaccga cgatacgagt gggaccgtgg 2401 tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca 2461 gaccgacgat acgagtggga ccgtggtccc agtctgatta tcagaccgac gatacaagtg 2521 gaacagtggg cccagagaga atattcaggc cagttatgct ttctggcctg taacaaagga 2581 cattaagtaa agacagataa acgtagacta aaacgtggtc gcatcagggt gctggctttt 2641 caagttcctt aagaatggcc tcaattttct ctatacactc agttggaaca cgagacctgt 2701 ccaggttaag caccatttta tcgcccttat acaatactgt cgctccagga gcaaactgat 2761 gtcgtgagct taaacttgtt cttgatgcag atgacgtttt aagcacagaa gttaaaagag 2821 tgataacttc ttcagcttca aatatcaccc cagctttttt ctgctcatga aggttagatg 2881 cctgctgctt aagtaattcc tctttatctg taaaggcttt ttgaagtgca tcacctgacc 2941 gggcagatag ttcaccgggg tgagaaaaaa gagcaacaac tgatttaggc aatttggcgg 3001 tgttgataca gcgggtaata atcttacgtg aaatattttc cgcatcagcc agcgcagaaa 3061 tatttccagc aaattcattc tgcaatcggc ttgcataacg ctgaccacgt tcataagcac 3121 ttgttgggcg ataatcgtta cccaatctgg ataatgcagc catctgctca tcatccagct 3181 cgccaaccag aacacgataa tcactttcgg taagtgcagc agctttacga cggcgactcc 3241 catcggcaat ttctatgaca ccagatactc ttcgaccgaa cgccggtgtc tgttgaccag 3301 tcagtagaaa agaagggatg agatcatcca gtgcgtcctc agtaagcagc tcctggtcac 3361 gttcattacc tgaccatacc cgagaggtct tctcaacact atcaccccgg agcacttcaa 3421 gagtaaactt cacatcccga ccacatacag gcaaagtaat ggcattaccg cgagccatta 3481 ctcctacgcg cgcaattaac gaatccacca tcggggcagc tggtgtcgat aacgaagtat 3541 cttcaaccgg ttgagtattg agcgtatgtt ttggaataac aggcgcacgc ttcattatct 3601 aatctcccag cgtggtttaa tcagacgatc gaaaatttca ttgcagacag gttcccaaat 3661 agaaagagca tttctccagg caccagttga agagcgttga tcaatggcct gttcaaaaac 3721 agttctcatc cggatctgac ctttaccaac ttcatccgtt tcacgtacaa cattttttag 3781 aaccatgctt ccccaggcat cccgaatttg ctcctccatc cacggggact gagagccatt 3841 actattgctg tatttggtaa gcaaaatacg tacatcaggc tcgaaccctt taagatcaac 3901 gttcttgagc agatcacgaa gcatatcgaa aaattgcagt gcggaggtgt agtcaaacaa 3961 ctcagcaggc gtgggaacaa tcagcacatc agcagcacat acgacattaa tcgtgccgat 4021 acccaggtta ggcgcgctgt caataactat gacatcatag tcatgagcaa cagtttcaat 4081 ggccagtcgg agcatcaggt gtggatcggt gggcagttta ccttcatcaa atttgcccat 4141 taactcagtt tcaatacggt gcagagccag acaggaagga ataatgtcaa gccccggcca 4201 gcaagtgggc tttattgcat aagtgacatc gtccttttcc ccaagataga aaggcaggag 4261 agtgtcttct gcatgaatat gaagatctgg tacccatccg tgatacattg aggctgttcc 4321 ctgggggtcg ttaccttcca cgagcaaaac acgtagcccc ttcagagcca gatcctgagc 4381 aagatgaaca gaaactgagg ttttgtaaac gccaccttta tgggcagcaa ccccgatcac 4441 cggtggaaat acgtcttcag cacgtcgcaa tcgcgtacca aacacatcac gcatatgatt 4501 aatttgttca attgtataac caacacgttg ctcaacccgt cctcgaattt ccatatccgg 4561 gtgcggtagt cgccctgctt tctcggcatc tctgatagcc tgagaagaaa ccccaactaa 4621 atccgctgct tcacctattc tccagcgccg ggttattttc ctcgcttccg ggctgtcatc 4681 attaaactgt gcaatggcga tagccttcgt catttcatga ccagcgttta tgcactggtt 4741 aagtgtttcc atgagtttca ttctgaacat cctttaatca ttgctttgcg tttttttatt 4801 aaatcttgca atttactgca aagcaacaac aaaatcgcaa agtcatcaaa aaaccgcaaa 4861 gttgtttaaa ataagagcaa cactacaaaa ggagataaga agagcacata cctcagtcac 4921 ttattatcac tagcgctcgc cgcagccgtg taaccgagca tagcgagcga actggcgagg 4981 aagcaaagaa gaactgttct gtcagatagc tcttacgctc agcgcaagaa gaaatatcca 5041 ccgtgggaaa aactccaggt agaggtacac acgcggatag ccaattcaga gtaataaact 5101 gtgataatca accctcatca atgatgacga actaaccccc gatatcaagt cacatgacga 5161 agggaaagag aaggaaatca actgtgacaa actgccctca aatttggctt ccttaaaaat 5221 tacagttcaa aaagtatgag aaaatccatg caggctgaag gaaacagcaa aactgtgaca 5281 aattaccctc agtaggtcag aacaaatgtg acgaaccacc ctcaaatctg tgacagataa 5341 ccctcagact atcctgtcgt catggaagtg atatcgcgga aggaaaatac gatatgagtc 5401 gtctggcggc ctttcttttt ctcaatgtat gagaggcgca ttggagttct gctgttgatc 5461 tcattaacac agacttgcag gaagcggcgg cggaagtcag gcatacgctg gtaactttga 5521 ggcagctggt aacgctctat gatccagtcg attttcagag agacgatgcc tgagccatcc 5581 ggcttacgat actgacacag ggattcgtat aaacgcatgg catacggatt ggtgatttct 5641 tttgtttcac taagccgaaa ctgcgtaaac cggttctgta acccgataaa gaagggaatg 5701 agatatgggt tgatatgtac actgtaaagc cctctggatg gactgtgcgc acgtttgata 5761 aaccaaggaa aagattcata gcctttttca tcgccggcat cctcttcagg gcgataaaaa 5821 accacttcct tccccgcgaa actcttcaat gcctgccgta tatccttact ggcttccgca 5881 gaggtcaatc cgaatatttc agcatattta gcaacatgga tctcgcagat accgtcatgt 5941 tcctgtaggg tgccatcaga ttttctgatc tggtcaacga acagatacag catacgtttt 6001 tgatcccggg agagactata tgccgcctca gtgaggtcgt ttgactggac gattcgcggg 6061 ctatttttac gtttcttgtg attgataacc gctgtttccg ccatgacaga tccatgtgaa 6121 gtgtgacaag tttttagatt gtcacactaa ataaaaaaga gtcaataagc agggataact 6181 ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacag ggttaagggc aatttgtcac 6241 agacaggact gtcatttgag ggtgatttgt cacactgaaa gggcaatttg tcacaacacc 6301 ttctgtagaa ccagcatgga taaaggccta caaggcgctc taaaaaagaa gatctaaaaa 6361 ctataaaaaa aataattata aaaatatccc cgtggataag tggataaccc caagggaagt 6421 tttttcaggc atcgtgtgta agcagaatat ataagtgctg ttccctggtg cttcctcgct 6481 cactcgatac tagagtccgg caaaaaaacg ggcaaggtgt caccaccctg ccctttttct 6541 ttaaaaccga aaagattact tcgcgtttac tagagtgcca cctgacgtct aagaatacta 6601 gagaaggaat attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc 6661 agtgagttga ttgctacgta atactagagt tagttagtta gtactagagc ccttagtgac 6721 tctactagag gaattcgcgg ccgcttctag agtactagag ggcttactaa aagccagata 6781 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac 6841 ccgaagtatg tcaaaaagag gtatgctatg cagtttaagg tttacaccta taaacgtgag 6901 agccgttatc gtctgtttgt ggacgtacag agtgatatta ttgacacgcc tgggcgtcgc 6961 atggtgattc ccctggccag cgcacgtctg ctttcagata aagtctcccg tgaactttac 7021 ccggtggtgc atatcgggga cgaaagctgg cgcatgatga ccaccgatat ggccagcgtg 7081 ccggtttccg tgatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa 7141 aacgccatta acctgatgtt ctggggaata taataatact agagcccgta gaaaagatca 7201 aaagatcttc ttgagatcct ttttttctgc gcgtaatctg ctacttgcaa acaaaaaaac 7261 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 7321 taactggctt tagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 7381 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 7441 cagtagctac tgccagtagc gataagtcgt gtcttaccgg gttggattca agacgatagt 7501 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 7561 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 7621 ttcccgaagg gagaaaggcg gacaggtatc tggtaagcgg cagggtcgga acaggagagc 7681 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 7741 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 7801 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacat //