promoter_anderson
1
//promoter/anderson
BBa_K1585106
1
BBa_K1585106
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:01:39Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441696
1
BBa_J23106
component2441698
1
BBa_K1585999
annotation2441696
1
BBa_J23106
range2441696
1
1
35
annotation2441698
1
BBa_K1585999
range2441698
1
44
78
BBa_K777128
1
BBa_K777128
Constitutive J23106 promoter
Team Goettingen
2012-09-19T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23106 J23106]) and the plasmid backbone pSB1C3.
* No specific design considerations
* pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2204868
1
J23106
range2204868
1
2
36
annotation2204869
1
constitutive promoter J23106
range2204869
1
2
36
BBa_J23115
1
BBa_J23115
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
In stock
true
Later
N/A
Later
true
BBa_K777132
1
BBa_K777132
Constitutive J23114 promoter
Team Goettingen
2012-09-19T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23114 J23114]) and the plasmid backbone pSB1C3.
* No specific design considerations
* pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2204948
1
J23114
range2204948
1
2
36
annotation2204949
1
constitutive promoter J23114
range2204949
1
2
36
BBa_J23106
1
BBa_J23106
constitutive promoter family member
John Anderson
2006-08-13T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
Later
N/A
Isolated from library of promoters
true
BBa_K777108
1
BBa_K777108
flhDC operon under the control of constitutive promoter J23114
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
In stock
false
[[Image:Table_K777101-K777108_new.jpg|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''flhDC'' operon downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
<br>Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
*The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer.
*''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'
**Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' <br> (the capitalized "C" induces the mutation for removal of the PstI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2187126
1
BBa_K777100
component2187118
1
BBa_J23114
annotation2187126
1
BBa_K777100
range2187126
1
42
910
annotation2187118
1
BBa_J23114
range2187118
1
1
35
BBa_K1585117
1
BBa_K1585117
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:23:31Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23116 J23116] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441711
1
BBa_J23117
component2441713
1
BBa_K1585999
annotation2441713
1
BBa_K1585999
range2441713
1
44
78
annotation2441711
1
BBa_J23117
range2441711
1
1
35
BBa_K1585104
1
BBa_K1585104
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T03:26:03Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23104 J23104] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441422
1
BBa_K1585999
component2441420
1
BBa_J23104
annotation2441422
1
BBa_K1585999
range2441422
1
44
78
annotation2441420
1
BBa_J23104
range2441420
1
1
35
BBa_K777006
1
BBa_K777006
Tar receptor under the control of constitutive promoter J23112
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
It's complicated
false
[[Image:Table_K777001-K777008_new.png|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23112 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189398
1
BBa_K777000
component2189394
1
BBa_J23112
annotation2189394
1
BBa_J23112
range2189394
1
1
35
annotation2189398
1
BBa_K777000
range2189398
1
42
1703
BBa_J23108
1
BBa_J23108
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
In stock
true
Later
N/A
Later
true
BBa_K777118
1
BBa_K777118
motB under the control of constitutive promoter J23100
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
Not in stock
false
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
* ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3'
** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2190664
1
BBa_K777117
component2190662
1
BBa_J23100
annotation2190662
1
BBa_J23100
range2190662
1
1
35
annotation2190664
1
BBa_K777117
range2190664
1
42
968
BBa_K777124
1
BBa_K777124
yhjH under the control of constitutive promoter J23114
Team Goettingen
2012-09-21T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
Not in stock
false
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants.
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2193024
1
BBa_K777121
component2193022
1
BBa_J23114
annotation2193024
1
BBa_K777121
range2193024
1
42
809
annotation2193022
1
BBa_J23114
range2193022
1
1
35
BBa_K777001
1
BBa_K777001
Tar receptor under the control of constitutive promoter J23100
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
It's complicated
true
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate.
<br>Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189126
1
BBa_K777000
component2189122
1
BBa_J23100
annotation2189126
1
BBa_K777000
range2189126
1
42
1703
annotation2189122
1
BBa_J23100
range2189122
1
1
35
BBa_J23107
1
BBa_J23107
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
false
_52_
0
483
95
It's complicated
true
Later
N/A
Later
true
BBa_K777127
1
BBa_K777127
Constitutive J23105 promoter
Team Goettingen
2012-09-19T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23105 J23105]) and the plasmid backbone pSB1C3.
No specific design considerations
pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2204849
1
constitutive promoter J23105
range2204849
1
2
36
annotation2204848
1
J23105
range2204848
1
2
36
BBa_K777005
1
BBa_K777005
Tar receptor under the control of constitutive promoter J23109
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
It's complicated
false
[[Image:Table_K777001-K777008_new.png|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23109 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189368
1
BBa_J23109
component2189372
1
BBa_K777000
annotation2189372
1
BBa_K777000
range2189372
1
42
1703
annotation2189368
1
BBa_J23109
range2189368
1
1
35
BBa_K777008
1
BBa_K777008
Tar receptor under the control of constitutive promoter J23114
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
It's complicated
false
[[Image:Table_K777001-K777008_new.png|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23114 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189404
1
BBa_J23114
component2189408
1
BBa_K777000
annotation2189408
1
BBa_K777000
range2189408
1
42
1703
annotation2189404
1
BBa_J23114
range2189404
1
1
35
BBa_K1585110
1
BBa_K1585110
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:09:56Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441701
1
BBa_K1585999
component2441699
1
BBa_J23110
annotation2441701
1
BBa_K1585999
range2441701
1
44
78
annotation2441699
1
BBa_J23110
range2441699
1
1
35
BBa_K777123
1
BBa_K777123
yhjH under the control of constitutive promoter J23112
Team Goettingen
2012-09-21T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
Not in stock
false
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants.
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2192772
1
BBa_J23112
component2192774
1
BBa_K777121
annotation2192774
1
BBa_K777121
range2192774
1
42
809
annotation2192772
1
BBa_J23112
range2192772
1
1
35
BBa_K777104
1
BBa_K777104
flhDC operon under the control of constitutive promoter J23106
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
In stock
false
[[Image:Table_K777101-K777108_new.jpg|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''flhDC'' operon downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
<br>Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
* The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer
* ''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'
** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3'<br>(the capitalized "C" induces the mutation for removal of the PstI site)
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2187047
1
BBa_K777100
component2187039
1
BBa_J23106
annotation2187039
1
BBa_J23106
range2187039
1
1
35
annotation2187047
1
BBa_K777100
range2187047
1
42
910
BBa_K823013
1
BBa_K823013
Anderson promoter J23117
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:51:22Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23117 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182544
1
-35 box
range2182544
1
1
6
annotation2182543
1
J23117
range2182543
1
1
35
annotation2182545
1
-10 box
range2182545
1
24
29
BBa_K823014
1
BBa_K823014
Anderson promoter J23118
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:51:43Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23118 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182546
1
J23118
range2182546
1
1
35
annotation2182548
1
-10 box
range2182548
1
24
29
annotation2182547
1
-35 box
range2182547
1
1
6
BBa_K823004
1
BBa_K823004
Anderson promoter J23100
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:43:33Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23100 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182508
1
-10 box
range2182508
1
24
29
annotation2182506
1
J23100
range2182506
1
1
35
annotation2182507
1
-35 box
range2182507
1
1
6
BBa_K418000
1
CP1LacPI1
IPTG inducible Lac promoter cassette
Northwestern University iGEM 2010
2010-10-11T11:00:00Z
2015-05-08T01:12:28Z
false
true
_532_
0
6657
9
Not in stock
false
constituitive promoter + LacPI
blah
From assembly of kit parts.
false
component2263742
1
BBa_Q01121
component2263723
1
BBa_J23100
annotation2263723
1
BBa_J23100
range2263723
1
1
35
annotation2263742
1
BBa_Q01121
range2263742
1
44
1416
BBa_K1330002
1
BBa_K1330002
Constitutive promoter (J23105)
Kristian Jensen
2014-09-29T11:00:00Z
2015-05-08T01:09:55Z
false
false
_1705_
0
20126
9
In stock
false
t
t
t
false
BBa_J23103
1
BBa_J23103
constitutive promoter family member
John Anderson
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
replace later
N/A
isolated from library of promoters
true
BBa_K777122
1
BBa_K777122
yhjH under the control of constitutive promoter J23100
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
Not in stock
false
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''yhjH'' expression affect the motility of ''E. coli''.
<br><br>
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
** Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2191153
1
BBa_J23100
component2191157
1
BBa_K777121
component2191155
1
BBa_B0034
annotation2191153
1
BBa_J23100
range2191153
1
1
35
annotation2191157
1
BBa_K777121
range2191157
1
62
829
annotation2191155
1
BBa_B0034
range2191155
1
44
55
BBa_K777125
1
BBa_K777125
Constitutive J23100 promoter
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out J61002 and J23100) and the plasmid backbone pSB1C3.
* No specific design considerations
* pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2203710
1
J23100
range2203710
1
2
36
annotation2203711
1
constitutive promoter J23100
range2203711
1
2
36
BBa_K823007
1
BBa_K823007
Anderson promoter J23103
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:46:24Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23103 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182516
1
-35 box
range2182516
1
1
6
annotation2182517
1
-10 box
range2182517
1
24
29
annotation2182515
1
J23103
range2182515
1
1
35
BBa_K777111
1
BBa_K777111
fliC under the control of constitutive promoter J23112
Team Goettingen
2012-09-21T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Not in stock
false
The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
* The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
false
component2192728
1
BBa_K777109
component2192720
1
BBa_J23112
annotation2192720
1
BBa_J23112
range2192720
1
1
35
annotation2192728
1
BBa_K777109
range2192728
1
42
1538
BBa_K823008
1
BBa_K823008
Anderson promoter J23106
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:47:05Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182528
1
-35 box
range2182528
1
1
6
annotation2182530
1
J23106
range2182530
1
1
35
annotation2182529
1
-10 box
range2182529
1
24
29
BBa_K777004
1
BBa_K777004
Tar receptor under the control of constitutive promoter J23106
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
It's complicated
false
[[Image:Table_K777001-K777008_new.png|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23106 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189302
1
BBa_K777000
component2189298
1
BBa_J23106
annotation2189302
1
BBa_K777000
range2189302
1
42
1703
annotation2189298
1
BBa_J23106
range2189298
1
1
35
BBa_K777007
1
BBa_K777007
Tar receptor under the control of constitutive promoter J23113
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
In stock
false
[[Image:Table_K777001-K777008_new.png|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23113 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189403
1
BBa_K777000
component2189399
1
BBa_J23113
annotation2189399
1
BBa_J23113
range2189399
1
1
35
annotation2189403
1
BBa_K777000
range2189403
1
42
1703
BBa_J23105
1
BBa_J23105
constitutive promoter family member
John Anderson
2006-08-13T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
In stock
true
Later
N/A
Isolated from library of promoters
true
BBa_K1585105
1
BBa_K1585105
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:03:04Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441414
1
BBa_J23105
component2441416
1
BBa_K1585999
annotation2441416
1
BBa_K1585999
range2441416
1
44
78
annotation2441414
1
BBa_J23105
range2441414
1
1
35
BBa_K1468000
1
BBa_K1468000
pJ23104 + gene encoding ZsGreen
Pedro Luis Dorado Morales
2014-10-02T11:00:00Z
2015-05-08T01:10:38Z
false
false
_1847_
0
11756
9
It's complicated
true
-
-
-
false
annotation2418060
1
BBa_E0040
range2418060
1
34
725
annotation2418058
1
BBa_J23104
range2418058
1
1
33
annotation2418064
1
STOP
range2418064
1
725
725
annotation2418062
1
ZsGreeen
range2418062
1
34
725
annotation2418810
1
BBa_K1468000
range2418810
1
1
725
annotation2418059
1
ZsGreen protein
range2418059
1
34
725
annotation2418061
1
START
range2418061
1
34
36
annotation2418030
1
J23104p
range2418030
1
1
33
BBa_J23118
1
BBa_J23118
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
Later
N/A
Later
true
BBa_K292000
1
BBa_K292000
Double terminator + constitutive promoter
David Charoy
2009-10-17T11:00:00Z
2015-05-08T01:11:49Z
false
true
_394_
0
5212
9
It's complicated
false
This part contains a double terminator and a constitutive prokaryotic promoter. It is a simple promoter to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used after to be added to a recombinant lambda phage to begin the transcription of synthetic genes inside the Lambda phage genome.
N/A
BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16)
BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04)
false
component2059163
1
BBa_J23100
component2059161
1
BBa_B0011
component2059157
1
BBa_B0012
annotation2059161
1
BBa_B0011
range2059161
1
50
95
annotation2059157
1
BBa_B0012
range2059157
1
1
41
annotation2059163
1
BBa_J23100
range2059163
1
104
138
BBa_K777129
1
BBa_K777129
Constitutive J23109 promoter
Team Goettingen
2012-09-19T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23109 J23109]) and the plasmid backbone pSB1C3.
* No specific design considerations
* pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2204888
1
J23109
range2204888
1
2
36
annotation2204889
1
constitutive promoter J23109
range2204889
1
2
36
BBa_K777112
1
BBa_K777112
fliC under the control of constitutive promoter J23114
Team Goettingen
2012-09-21T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Not in stock
false
The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''.
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
* The ''fliC'' gene was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2192746
1
BBa_J23114
component2192754
1
BBa_K777109
annotation2192746
1
BBa_J23114
range2192746
1
1
35
annotation2192754
1
BBa_K777109
range2192754
1
42
1538
BBa_K777130
1
BBa_K777130
Constitutive J23112 promoter
Team Goettingen
2012-09-19T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23112 J23112]) and the plasmid backbone pSB1C3.
* No specific design considerations
* pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2204908
1
J23112
range2204908
1
2
36
annotation2204909
1
constitutive promoter J23112
range2204909
1
2
36
BBa_K777102
1
BBa_K777102
flhDC operon under the control of constitutive promoter J23104
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
It's complicated
false
The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive flhDC expression affect the motility of E. coli.
* The PstI restriction site in the flhDC sequence was mutated to CTGCGG using the reverse primer
* flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'
** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3'<br>(the capitalized "C" induces the mutation for removal of the PstI site)
* The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2187014
1
BBa_K777100
component2187006
1
BBa_J23104
annotation2187014
1
BBa_K777100
range2187014
1
42
910
annotation2187006
1
BBa_J23104
range2187006
1
1
35
BBa_K777115
1
BBa_K777115
motA under the control of constitutive promoter J23112
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Not in stock
false
The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3'
**Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
* ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189728
1
BBa_K777113
component2189726
1
BBa_J23112
annotation2189728
1
BBa_K777113
range2189728
1
42
872
annotation2189726
1
BBa_J23112
range2189726
1
1
35
BBa_K1679004
1
BBa_K1679004
J23101 and RiboJ
Qikai Qin
2015-09-15T11:00:00Z
2015-09-18T06:26:36Z
false
false
_2097_
25072
21726
9
false
BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits from genetic context. This part can be used as a promoter-like parts.
We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part.
BBa_J23101
We synthesized RiboJ. Our reference is Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.
false
component2474697
1
BBa_K1679038
component2474696
1
BBa_J23101
annotation2474697
1
BBa_K1679038
range2474697
1
44
118
annotation2474696
1
BBa_J23101
range2474696
1
1
35
BBa_K418003
1
CP2LacPI1
IPTG inducible Lac promoter cassette
Northwestern University iGEM 2010
2010-10-15T11:00:00Z
2015-05-08T01:12:28Z
false
false
_532_
0
6938
9
It's complicated
true
N/A
N/A
N/A
false
component2263763
1
BBa_J23104
component2263782
1
BBa_Q01121
annotation2263782
1
BBa_Q01121
range2263782
1
44
1416
annotation2263763
1
BBa_J23104
range2263763
1
1
35
BBa_K2122000
1
BBa_K2122000
Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
Sannia Farrukh
2016-09-28T11:00:00Z
2016-10-18T01:26:30Z
false
false
_2591_
32074
32074
9
true
This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.
Coding Sequence from GenBank
false
component2517232
1
BBa_B0014
component2517222
1
BBa_I0500
component2517225
1
BBa_K2122150
component2517224
1
BBa_B0034
annotation2517232
1
BBa_B0014
range2517232
1
2307
2401
annotation2517222
1
BBa_I0500
range2517222
1
1
1210
annotation2517224
1
BBa_B0034
range2517224
1
1219
1230
annotation2517225
1
BBa_K2122150
range2517225
1
1237
2298
BBa_K1585119
1
BBa_K1585119
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:33:36Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23119 J23119] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441719
1
BBa_J23119
component2441721
1
BBa_K1585999
annotation2441719
1
BBa_J23119
range2441719
1
1
35
annotation2441721
1
BBa_K1585999
range2441721
1
44
78
BBa_J23112
1
BBa_J23112
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
false
_52_
0
483
95
Released HQ 2013
In stock
true
Later
N/A
Later
true
BBa_K1585103
1
BBa_K1585103
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T03:08:18Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441411
1
BBa_J23103
component2441413
1
BBa_K1585999
annotation2441413
1
BBa_K1585999
range2441413
1
44
78
annotation2441411
1
BBa_J23103
range2441411
1
1
35
BBa_K418002
1
CP1LacPI2
IPTG inducible Lac promoter cassette
Northwestern University iGEM 2010
2010-10-15T11:00:00Z
2015-05-08T01:12:28Z
false
false
_532_
0
6938
9
It's complicated
false
N/A
N/A
N/A
false
component2263762
1
BBa_Q04121
component2263743
1
BBa_J23100
annotation2263743
1
BBa_J23100
range2263743
1
1
35
annotation2263762
1
BBa_Q04121
range2263762
1
44
1414
BBa_K823011
1
BBa_K823011
Anderson promoter J23114
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:50:55Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23114 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182537
1
J23114
range2182537
1
1
35
annotation2182538
1
-35 box
range2182538
1
1
6
annotation2182539
1
-10 box
range2182539
1
24
29
BBa_K777110
1
BBa_K777110
fliC under the control of constitutive promoter J23100
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Not in stock
false
The ''fliC'' gene codes for flagellin. This protein is the subunit that forms the flagellar filament of ''E. coli''.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''fliC'' expression affect the motility or flagellum size of ''E. coli''.
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
* ''fliC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatggcacaagtcattaatacc-3'
** Rev: 5'-gtttcttcgaattcgcggccgcttctagttaaccctgGagcagagacagaacc-3' <br> (the capitalized "G" induces the mutation for removal of the third PstI site)
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
false
component2189303
1
BBa_J23100
component2189311
1
BBa_K777109
annotation2189303
1
BBa_J23100
range2189303
1
1
35
annotation2189311
1
BBa_K777109
range2189311
1
42
1538
BBa_K1585113
1
BBa_K1585113
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:09:36Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441704
1
BBa_K1585999
component2441702
1
BBa_J23113
annotation2441702
1
BBa_J23113
range2441702
1
1
35
annotation2441704
1
BBa_K1585999
range2441704
1
44
78
BBa_K1585102
1
BBa_K1585102
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:03:57Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23102 J23102] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441419
1
BBa_K1585999
component2441417
1
BBa_J23102
annotation2441417
1
BBa_J23102
range2441417
1
1
35
annotation2441419
1
BBa_K1585999
range2441419
1
44
78
BBa_K777106
1
BBa_K777106
flhDC operon under the control of constitutive promoter J23112
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
In stock
false
[[Image:Table_K777101-K777108_new.jpg|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''flhDC'' operon downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
<br>Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
*The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer.
*''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'
**Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' <br>(the capitalized "C" induces the mutation for removal of the PstI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2187094
1
BBa_K777100
component2187086
1
BBa_J23112
annotation2187094
1
BBa_K777100
range2187094
1
42
910
annotation2187086
1
BBa_J23112
range2187086
1
1
35
BBa_J23110
1
BBa_J23110
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
In stock
true
Later
N/A
Later
true
BBa_J23117
1
BBa_J23117
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
Later
N/A
Later
true
BBa_K823005
1
BBa_K823005
Anderson promoter J23101
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:44:25Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182510
1
-35 box
range2182510
1
1
6
annotation2182509
1
J23101
range2182509
1
1
35
annotation2182511
1
-10 box
range2182511
1
24
29
BBa_K1585116
1
BBa_K1585116
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:19:47Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441710
1
BBa_K1585999
component2441708
1
BBa_J23116
annotation2441708
1
BBa_J23116
range2441708
1
1
35
annotation2441710
1
BBa_K1585999
range2441710
1
44
78
BBa_K1468002
1
BBa_K1468002
pJ23110 + gene encoding ZsGreen
Pedro Luis Dorado Morales
2014-10-02T11:00:00Z
2015-05-08T01:10:38Z
false
false
_1847_
0
11756
9
It's complicated
false
-
-
-
false
BBa_J23114
1
BBa_J23114
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
Later
N/A
Later
true
BBa_K777101
1
BBa_K777101
flhDC operon under the control of constitutive promoter J23100
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
In stock
false
The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
Here we used a set of 8 constitutive promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect motility in ''E. coli''.
*The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer.
*''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'<br>
**Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' <br>(the capitalized "C" induces the mutation for removal of the PstI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23100 information was from partsregistry and physical DNA from the 2012 distribution kit.
false
component2186537
1
BBa_K777100
component2186529
1
BBa_J23100
annotation2186529
1
BBa_J23100
range2186529
1
1
35
annotation2186537
1
BBa_K777100
range2186537
1
42
910
BBa_K1585115
1
BBa_K1585115
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:13:08Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441705
1
BBa_J23115
component2441707
1
BBa_K1585999
annotation2441705
1
BBa_J23115
range2441705
1
1
35
annotation2441707
1
BBa_K1585999
range2441707
1
44
78
BBa_J23116
1
BBa_J23116
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
false
_52_
0
483
95
In stock
true
Later
N/A
Later
true
BBa_K1585100
1
BBa_K1585100
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-23T11:00:00Z
2015-08-26T11:28:17Z
false
false
_2002_
24474
24474
9
Not in stock
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same.
We choosed part BBa_R0010 because we assumed older parts would be reliable.
Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
false
component2436913
1
BBa_K1585999
component2436912
1
BBa_J23100
annotation2436913
1
BBa_K1585999
range2436913
1
44
78
annotation2436912
1
BBa_J23100
range2436912
1
1
35
BBa_K292001
1
BBa_K292001
Double terminator + Constitutive promoter + Strong RBS
David Charoy
2009-10-17T11:00:00Z
2015-05-08T01:11:49Z
false
false
_394_
0
5212
9
It's complicated
false
This part contains a double terminator and a constitutive prokaryotic promoter and a strong RBS (BBa_B0014 + BBa_J23100 + BBa_B0030). It is a simple promoter in the goals to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used when adding to a recombinant lambda phage and to begin the transcription of synthetic genes inside the Lambda phage genome.
N/A
BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16).<br>
BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04.)<br>
BBa_B0030 : Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.
false
component2052039
1
BBa_J23100
component2052041
1
BBa_B0030
component2052037
1
BBa_B0011
component2052033
1
BBa_B0012
annotation2052037
1
BBa_B0011
range2052037
1
50
95
annotation2052039
1
BBa_J23100
range2052039
1
104
138
annotation2052041
1
BBa_B0030
range2052041
1
147
161
annotation2052033
1
BBa_B0012
range2052033
1
1
41
BBa_K777119
1
BBa_K777119
motB under the control of constitutive promoter J23112
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
Not in stock
false
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
<br><br>
* ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3'
** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2190796
1
BBa_J23112
component2190798
1
BBa_K777117
annotation2190798
1
BBa_K777117
range2190798
1
42
968
annotation2190796
1
BBa_J23112
range2190796
1
1
35
BBa_K256002
1
BBa_K256002
J23101:GFP
iGEM09_NTU-Singapore
2009-10-15T11:00:00Z
2015-05-08T01:11:40Z
false
false
_359_
0
4864
9
It's complicated
true
GFP Reporter
--
--
false
component2056720
1
BBa_B0010
component2056717
1
BBa_B0034
component2056722
1
BBa_B0012
component2056715
1
BBa_J23101
component2056719
1
BBa_E0040
annotation2056722
1
BBa_B0012
range2056722
1
878
918
annotation2056720
1
BBa_B0010
range2056720
1
790
869
annotation2056719
1
BBa_E0040
range2056719
1
62
781
annotation2056717
1
BBa_B0034
range2056717
1
44
55
annotation2056715
1
BBa_J23101
range2056715
1
1
35
BBa_K777120
1
BBa_K777120
motB under the control of constitutive promoter J23114
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
Not in stock
false
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motB'' expression affect the motility of ''E. coli''.
<br><br>
* ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3'
** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2190799
1
BBa_J23114
component2190801
1
BBa_K777117
annotation2190801
1
BBa_K777117
range2190801
1
42
968
annotation2190799
1
BBa_J23114
range2190799
1
1
35
BBa_K823006
1
BBa_K823006
Anderson promoter J23102
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:45:05Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23102 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182514
1
-10 box
range2182514
1
24
29
annotation2182513
1
-35 box
range2182513
1
1
6
annotation2182512
1
J23102
range2182512
1
1
35
BBa_K1585101
1
BBa_K1585101
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-23T11:00:00Z
2015-09-01T03:27:19Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same.
We choosed part BBa_R0010 because we assumed older parts would be reliable.
Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
false
component2436906
1
BBa_J23101
component2436907
1
BBa_K1585999
annotation2436906
1
BBa_J23101
range2436906
1
1
35
annotation2436907
1
BBa_K1585999
range2436907
1
44
78
BBa_K1824896
1
BBa_K1824896
J23100 + RBS
Xinhao Wang
2015-09-10T11:00:00Z
2015-09-11T09:57:14Z
false
false
_2250_
26000
26000
9
false
Constitutive promoter J23100 plus RBS.
no special concern
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. B0030 is a strong RBS based on Ron Weiss thesis
false
component2452343
1
BBa_B0030
component2452340
1
BBa_J23100
component2452345
1
BBa_K1824890
component2452341
1
BBa_K1824889
annotation2452343
1
BBa_B0030
range2452343
1
60
74
annotation2452341
1
BBa_K1824889
range2452341
1
44
51
annotation2452345
1
BBa_K1824890
range2452345
1
83
88
annotation2452340
1
BBa_J23100
range2452340
1
1
35
BBa_J23109
1
BBa_J23109
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
In stock
true
Later
N/A
Later
true
BBa_K777003
1
BBa_K777003
Tar receptor under the control of constitutive promoter J23105
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
It's complicated
false
[[Image:Table_K777001-K777008_new.png|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23105 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23105 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189293
1
BBa_J23105
component2189297
1
BBa_K777000
annotation2189293
1
BBa_J23105
range2189293
1
1
35
annotation2189297
1
BBa_K777000
range2189297
1
42
1703
BBa_K823010
1
BBa_K823010
Anderson promoter J23113
Korinna Kraft
2012-09-07T11:00:00Z
2015-07-07T11:47:51Z
false
false
_1081_
4206
12081
9
Released HQ 2013
In stock
false
Anderson promoter J23113 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
no considerations
Partsregistry
false
annotation2182536
1
-10 box
range2182536
1
24
29
annotation2182534
1
J23113
range2182534
1
1
35
annotation2182535
1
-35 box
range2182535
1
1
6
BBa_K777105
1
BBa_K777105
flhDC operon under the control of constitutive promoter J23109
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
In stock
false
[[Image:Table_K777101-K777108_new.jpg|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''flhDC'' operon downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
<br>Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
*The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer.
*''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'
**Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' <br>(the capitalized "C" induces the mutation for removal of the PstI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2187048
1
BBa_J23109
component2187056
1
BBa_K777100
annotation2187056
1
BBa_K777100
range2187056
1
42
910
annotation2187048
1
BBa_J23109
range2187048
1
1
35
BBa_K1468001
1
BBa_K1468001
pJ23104 + gene encoding AsRed2
Pedro Luis Dorado Morales
2014-10-02T11:00:00Z
2015-05-08T01:10:38Z
false
false
_1847_
0
11756
9
It's complicated
false
-
-
-
false
BBa_J23104
1
BBa_J23104
constitutive promoter family member
John Anderson
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
false
false
_52_
0
483
95
In stock
true
replace later
N/A
isolated from library of promoters
true
BBa_K777002
1
BBa_K777002
Tar receptor under the control of constitutive promoter J23104
Bianca Genenncher
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
In stock
false
[[Image:Table_K777001-K777008_new.png|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''Tar'' gene downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
<br> <br> <br>
* Genomic sequence was amplified using the following primers.
** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA.
*** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3??
*** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3??
<br>
* One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
** Primers QuikChange (QC): Primers were provided by SIGMA.
*** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3??
*** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3??<br>(the uncapitalized letter induces the mutation for removal of the XbaI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189242
1
BBa_K777000
component2189238
1
BBa_J23104
annotation2189242
1
BBa_K777000
range2189242
1
42
1703
annotation2189238
1
BBa_J23104
range2189238
1
1
35
BBa_J23100
1
BBa_J23100
constitutive promoter family member
John Anderson
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
Replace later
N/A
Isolated from library of promoters
true
BBa_J23113
1
BBa_J23113
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
Later
N/A
Later
true
BBa_J23102
1
BBa_J23102
constitutive promoter family member
John Anderson
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
replace later
N/A
isolated from library of promoters
true
BBa_K2122100
1
BBa_K2122100
Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter
Sannia Farrukh
2016-09-28T11:00:00Z
2016-10-18T01:30:37Z
false
false
_2591_
32074
32074
9
false
This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
A constitutive promoter was added to the sequence
Coding Sequence from GenBank
false
component2518015
1
BBa_B0034
component2518023
1
BBa_B0014
component2518013
1
BBa_J23100
component2518016
1
BBa_K2122150
annotation2518023
1
BBa_B0014
range2518023
1
1132
1226
annotation2518015
1
BBa_B0034
range2518015
1
44
55
annotation2518016
1
BBa_K2122150
range2518016
1
62
1123
annotation2518013
1
BBa_J23100
range2518013
1
1
35
BBa_K777107
1
BBa_K777107
flhDC operon under the control of constitutive promoter J23113
Team Goettingen
2012-09-18T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
In stock
false
[[Image:Table_K777101-K777108_new.jpg|thumb|240px|right|'''Fig. 1:''' Overview of BioBricks containing the ''flhDC'' operon downstream of Anderson promoters. (Activity according to Berkeley 2006)]]
The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes.
<br>Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
*The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer.
*''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'
**Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' <br>(the capitalized "C" induces the mutation for removal of the PstI site)
*The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
*J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2187095
1
BBa_J23113
component2187103
1
BBa_K777100
annotation2187095
1
BBa_J23113
range2187095
1
1
35
annotation2187103
1
BBa_K777100
range2187103
1
42
910
BBa_J23119
1
BBa_J23119
constitutive promoter family member
John Anderson
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
false
Later
N/A
Overlap extension of synthetic oligonucleotides
true
BBa_J23101
1
BBa_J23101
constitutive promoter family member
John Anderson
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
Released HQ 2013
In stock
true
later
N/A
later
true
BBa_K1585118
1
BBa_K1585118
Anderson Promoter with lacI binding site
Tobias Schwanemann
2015-08-31T11:00:00Z
2015-09-01T02:31:42Z
false
false
_2002_
24474
24474
9
false
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
Assambly of [http://parts.igem.org/Part:BBa_J23118 J23118] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
false
component2441717
1
BBa_K1585999
component2441715
1
BBa_J23118
annotation2441715
1
BBa_J23118
range2441715
1
1
35
annotation2441717
1
BBa_K1585999
range2441717
1
44
78
BBa_K777126
1
BBa_K777126
Constitutive J23104 promoter
Team Goettingen
2012-09-19T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23100 J23100]) and the plasmid backbone pSB1C3.
No specific design considerations
pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2204828
1
J23104
range2204828
1
2
36
annotation2204829
1
constitutive promoter J23104
range2204829
1
2
36
BBa_J23111
1
BBa_J23111
constitutive promoter family member
John Anderson
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
false
true
_52_
0
483
95
In stock
true
Later
N/A
Later
true
BBa_K777131
1
BBa_K777131
Constitutive J23113 promoter
Team Goettingen
2012-09-19T11:00:00Z
2015-05-08T01:13:17Z
false
false
_1029_
0
12106
9
It's complicated
false
[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [http://partsregistry.org/Part:BBa_J61002 J61002] and [http://partsregistry.org/Part:BBa_J23113 J23113]) and the plasmid backbone pSB1C3.
* No specific design considerations
* pSB1C3 and the Anderson promoters were taken from the distribution kit 2012. (part J61002 with different constitutive promoters and pSB1C3)
false
annotation2204929
1
constitutive promoter J23113
range2204929
1
2
36
annotation2204928
1
J23113
range2204928
1
2
36
BBa_K777116
1
BBa_K777116
motA under the control of constitutive promoter J23114
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Not in stock
false
The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3'
**Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
* ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189732
1
BBa_J23114
component2189734
1
BBa_K777113
annotation2189734
1
BBa_K777113
range2189734
1
42
872
annotation2189732
1
BBa_J23114
range2189732
1
1
35
BBa_K777000
1
BBa_K777000
Tar receptor
Bianca Genenncher
2012-09-10T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
11946
9
In stock
It's complicated
false
The transmembrane receptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate.
One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
The part was amplified from genomic DNA of <i>E. coli</i> str. K-12 substr. DH10B chromosome (NC_010473.1).
false
annotation2182660
1
Tar coding region
range2182660
1
1
1662
annotation2182661
1
TGA
range2182661
1
1660
1662
annotation2182659
1
ATG
range2182659
1
1
3
BBa_K777114
1
BBa_K777114
motA under the control of constitutive promoter J23100
Team Goettingen
2012-09-20T11:00:00Z
2015-05-08T01:13:16Z
false
false
_1029_
0
12106
9
Not in stock
false
The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
<br>
Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''.
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
**Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3'
**Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3'
* ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
* J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
false
component2189705
1
BBa_J23100
component2189707
1
BBa_K777113
annotation2189705
1
BBa_J23100
range2189705
1
1
35
annotation2189707
1
BBa_K777113
range2189707
1
42
872