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Public
Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Unknown)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Public
iGEM to SBOL conversion
igem2sbol Version 1 (Activity)
Conversion of the iGEM parts registry to SBOL2.1
Public
SyBiOntKB to SBOL conversion
SyBiOntKB2sbol Version 1 (Activity)
Conversion of the Synthetic Biology Ontology knowledge base to SBOL 2.1
Public
SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Public
BBa_E0430
BBa_E0430 Version 1 (Component)
EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
Public
BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
Public
BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
Public
NLS+Term
BBa_K165018 Version 1 (Component)
SV40 nuclear localization sequence + ADH1 transcription terminator
Public
P22 attP
BBa_I11033 Version 1 (Component)
P22 ''attP''
Public
BBa_I732820
BBa_I732820 Version 1 (Component)
LacI (RBS+, TERM+)
Public
BBa_E0432
BBa_E0432 Version 1 (Component)
EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)
Public
BBa_P0455
BBa_P0455 Version 1 (Component)
RBS.Lambda cI LVA+.Term
Public
BBa_K309020
BBa_K309020 Version 1 (Component)
eCFP (for use in C. elegans chassis)
Public
BBa_K309012
BBa_K309012 Version 1 (Component)
Unc-54 3' UTR (for use in C. elegans chassis)
Public
HutP
BO_10864 Version 1 (Component)

Public
SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Public
BBa_K516022
BBa_K516022 Version 1 (Component)
AiiA protein generator TERM+ RBS+(B0032)
Public
BBa_I732017
BBa_I732017 Version 1 (Component)
lacZ full-length (RBS+ TERM-)
Public
BBa_K081009
BBa_K081009 Version 1 (Component)
lasI protein generator (TERM-)
Public
BBa_K081008
BBa_K081008 Version 1 (Component)
luxI protein generator (TERM-)
Public
BBa_K617004
BBa_K617004 Version 1 (Component)
Lambda attP P'OP
Public
BBa_K516030
BBa_K516030 Version 1 (Component)
mRFP protein generator TERM+ RBS+(B0030)
Public
SBOLDesigner CAD Tool
SBOLDesigner Version 2.2 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Public
BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency
Public
BBa_K876057
BBa_K876057 Version 1 (Component)
pTetR-lasR-Term-Term
Public
clyA_Nter
BBa_K257007 Version 1 (Component)
ClyA_Fusion_N-Term
Public
COX3 termi
BBa_K809005 Version 1 (Component)
Terminator of Yeast mitochondrial gene COX3
Public
BBa_K415000
BBa_K415000 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term
Public
BBa_M0100
BBa_M0100 Version 1 (Component)
tRNA_Arg5 to use as transcriptional reporter
Public
BBa_K876060
BBa_K876060 Version 1 (Component)
Term-Term-plasR-RBS-CFP-Term-Term
Public
BBa_K257002
BBa_K257002 Version 1 (Component)
ClyA_Fusion_C-Term
Public
BBa_K876069
BBa_K876069 Version 1 (Component)
Term-Term-pLuxR-TetR
Public
BBa_K876067
BBa_K876067 Version 1 (Component)
Term-Term-pLac-LuxR
Public
hutP
BO_2806 Version 1 (Component)

Public
BBa_K876068
BBa_K876068 Version 1 (Component)
Term-Term-pLsrA-LacI
Public
attP
BBa_K1463040 Version 1 (Component)
PhiC31 attP site
Public
YttP
BO_10887 Version 1 (Component)

Public
yerS
BO_32784 Version 1 (Component)

Public
YerS
BO_26301 Version 1 (Component)

Public
yttP
BO_30757 Version 1 (Component)

Public
BBa_K652004
BBa_K652004 Version 1 (Component)
RBS-ZeAFP-Term
Public
BBa_K876063
BBa_K876063 Version 1 (Component)
luxI-RBS-YFP-Term-Term
Public
BBa_I732835
BBa_I732835 Version 1 (Component)
ARL4A0604 (RBS+, TERM+)
Public
BBa_I732815
BBa_I732815 Version 1 (Component)
ARL4A0604 (RBS+, TERM-)
Public
BBa_I732018
BBa_I732018 Version 1 (Component)
lacZ alpha fragment (RBS+ TERM-)
Public
BBa_K876052
BBa_K876052 Version 1 (Component)
plasR-luxI-RBS-YFP-Term-Term
Public
BBa_K876073
BBa_K876073 Version 1 (Component)
pTetR-LasR-Term-Term-pLasR-LuxS-RBS-CFP-Term-Term-pLuxR-TetR
Public
BBa_K317030
BBa_K317030 Version 1 (Component)
<partinfo>P(J23102)-RBS(B0034)-pduP_GFP-Term(B0015) </partinfo>
Public
BBa_K081017
BBa_K081017 Version 1 (Component)
pcyA protein generator (TERM-)
Public
ShortLinke
BBa_K243004 Version 1 (Component)
Short Linker (Gly-Gly-Ser-Gly)
Showing 1 - 50 of 1162 result(s)
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