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Showing 51 - 100 of 2238 result(s)
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Public
IBMc381
IBMc381 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-H6-T
Public
IBMc385
IBMc385 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-160)-ALG-M86N-cat-Plux2-B32-M86C-S-mCherry*(161-236)-H6-T
Public
IBMc368
IBMc368 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-76)-TIG-ER-LBD-S-M86(77-154)-S-ECF20(102-193)-T
Public
IBMc367
IBMc367 Version 1 (Component)
pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
Public
IBMc388
IBMc388 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-174)-GGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(175-236)-H6-T
Public
Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Public
BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency
Public
Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
Public
BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
Public
BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7
Public
BBa_K1051006
BBa_K1051006 Version 1 (Component)
Saccharomycescerevisiae TBY-1 terminator with stopcodon
Public
BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB
Public
BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
Public
spacer
BBa_B0040 Version 1 (Component)
Spacer.1 (generic)
Public
Lock 1
BBa_J01010 Version 1 (Component)
Riboregulator Lock 1
Public
BBa_E0432
BBa_E0432 Version 1 (Component)
EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)
Public
P22 attP
BBa_I11033 Version 1 (Component)
P22 ''attP''
Public
BBa_I732820
BBa_I732820 Version 1 (Component)
LacI (RBS+, TERM+)
Public
NLS+Term
BBa_K165018 Version 1 (Component)
SV40 nuclear localization sequence + ADH1 transcription terminator
Public
gyrA_terminator
BO_4279 Version 1 (Component)

Public
rapJ_terminator
BO_4417 Version 1 (Component)

Public
purR-yabJ_terminator
BO_4313 Version 1 (Component)

Public
BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)
Public
BBa_K516022
BBa_K516022 Version 1 (Component)
AiiA protein generator TERM+ RBS+(B0032)
Public
BBa_P0455
BBa_P0455 Version 1 (Component)
RBS.Lambda cI LVA+.Term
Public
Key 1
BBa_J01008 Version 1 (Component)
Riboregulator key 1
Public
IBMc373_seq
IBMc373_seq Version 1 (Sequence)

Public
BBa_I712015
BBa_I712015 Version 1 (Component)
HIV-1 protease cleavage site
Public
Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.
Public
BBa_K2071969
BBa_K2071969 Version 1 (Component)
GLD1 is a general reductase, it is characterized in https://microbialcellfactories.biomedcentral.com
Public
BBa_K2071953
BBa_K2071953 Version 1 (Component)
A non-specific phosphotransferase, codon optimized for B.subtilis. Characterized in https://microbia
Public
Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Public
IBMc383_seq
IBMc383_seq Version 1 (Sequence)

Public
IBMc372_seq
IBMc372_seq Version 1 (Sequence)

Public
IBMc370_seq
IBMc370_seq Version 1 (Sequence)

Public
IBMc393_seq
IBMc393_seq Version 1 (Sequence)

Public
IBMc323_seq
IBMc323_seq Version 1 (Sequence)

Public
IBMc378_seq
IBMc378_seq Version 1 (Sequence)

Public
IBMc333_seq
IBMc333_seq Version 1 (Sequence)

Public
IBMc379_seq
IBMc379_seq Version 1 (Sequence)

Public
IBMc473_seq
IBMc473_seq Version 1 (Sequence)

Public
IBMc371_seq
IBMc371_seq Version 1 (Sequence)

Public
IBMc337_seq
IBMc337_seq Version 1 (Sequence)

Public
IBMc374_seq
IBMc374_seq Version 1 (Sequence)

Public
HutP
BO_10864 Version 1 (Component)

Public
BBa_K081009
BBa_K081009 Version 1 (Component)
lasI protein generator (TERM-)
Public
BBa_K617004
BBa_K617004 Version 1 (Component)
Lambda attP P'OP
Public
RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
Public
Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission
Public
BBa_K516030
BBa_K516030 Version 1 (Component)
mRFP protein generator TERM+ RBS+(B0030)
Showing 51 - 100 of 2238 result(s)
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