IBMc381
IBMc381 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-H6-T

IBMc385
IBMc385 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-160)-ALG-M86N-cat-Plux2-B32-M86C-S-mCherry*(161-236)-H6-T

IBMc368
IBMc368 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-76)-TIG-ER-LBD-S-M86(77-154)-S-ECF20(102-193)-T

IBMc367
IBMc367 Version 1 (Component)
pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3

IBMc388
IBMc388 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-174)-GGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(175-236)-H6-T

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

BBa_B0034
BBa_B0034 Version 1 (Component)
RBS (Elowitz 1999) -- defines RBS efficiency

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

BBa_E0422
BBa_E0422 Version 1 (Component)
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

BBa_B0012
BBa_B0012 Version 1 (Component)
TE from coliphageT7

BBa_K1051006
BBa_K1051006 Version 1 (Component)
Saccharomycescerevisiae TBY-1 terminator with stopcodon

BBa_B0010
BBa_B0010 Version 1 (Component)
T1 from E. coli rrnB

BBa_E0420
BBa_E0420 Version 1 (Component)
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)

spacer
BBa_B0040 Version 1 (Component)
Spacer.1 (generic)

Lock 1
BBa_J01010 Version 1 (Component)
Riboregulator Lock 1

BBa_E0432
BBa_E0432 Version 1 (Component)
EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)

P22 attP
BBa_I11033 Version 1 (Component)
P22 ''attP''

BBa_I732820
BBa_I732820 Version 1 (Component)
LacI (RBS+, TERM+)

NLS+Term
BBa_K165018 Version 1 (Component)
SV40 nuclear localization sequence + ADH1 transcription terminator

BBa_B0015
BBa_B0015 Version 1 (Component)
double terminator (B0010-B0012)

BBa_K516022
BBa_K516022 Version 1 (Component)
AiiA protein generator TERM+ RBS+(B0032)

BBa_P0455
BBa_P0455 Version 1 (Component)
RBS.Lambda cI LVA+.Term

Key 1
BBa_J01008 Version 1 (Component)
Riboregulator key 1

BBa_I712015
BBa_I712015 Version 1 (Component)
HIV-1 protease cleavage site

Composite Sequence Pull
Composite Version 1 (Activity)
When multiple sources where combined to make a new part.

BBa_K2071969
BBa_K2071969 Version 1 (Component)
GLD1 is a general reductase, it is characterized in https://microbialcellfactories.biomedcentral.com

BBa_K2071953
BBa_K2071953 Version 1 (Component)
A non-specific phosphotransferase, codon optimized for B.subtilis. Characterized in https://microbia

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K081009
BBa_K081009 Version 1 (Component)
lasI protein generator (TERM-)

BBa_K617004
BBa_K617004 Version 1 (Component)
Lambda attP P'OP

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

BBa_K516030
BBa_K516030 Version 1 (Component)
mRFP protein generator TERM+ RBS+(B0030)