GAL7

GenBank_CP003831_Region_178967_179986 Version 1

Component

Generated By: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_178967_179986_SBOLDesignerActivity/1
Date modified: 2022-06-22 19:42:10

Organism: Cryptococcus neoformans var. grubii H99



Types
DnaRegion

Roles
promoter

Sequences GenBank_CP003831_Region_178967_179986Sequence (Version 1)

Description

This is the galactose transferase promoter.

Notes

This promoter, GAL7 + 5'UTR, is repressible by glucose and inducible by galactose. The 3' primer used to amplify the GAL7 promoter region encopassed the first codon ATG. This ATG codon was, therefore, excluded from the promoter presented here, diminishing the size of this promoter from 1022 to 1020 bp.

References


Ruff, JA., Lodge, JK., Baker, LG., "Three galactose inducible promoters for use in C. neoformans var. grubii.", Fungal genetics and biology : FG & B, 2009, Jan 46(1): 9-16, PMID: 18952189., DOI: 10.1016/j.fgb.2008.10.003.

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