Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ronny Krashinsky
Date created: 2004-01-25 12:00:00
Date modified: 2015-08-31 04:07:25
Repressor, Zif23-GCN4 (RBS-, LVA+)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_C2000_sequence (Version 1) |
Description
Coding region for Zif23-GCN4. This protein is constructed out of fingers 2 and 3 from Zif268 fused with the leucine zipper domain from GCN4. The protein binds cooperatively as a dimer to a symmetric target DNA sequence in which each monomer targets 5'-GCGTGG-3'.Notes
As in [Wolfe00], the protein uses residues 364-412 of Zif268:BAC30748.1
The corresponding nucleotide sequence was taken from:
AK040925.1
As in [Wolfe00], the protein uses residues 250-281 of GCN4:
AAA34640.1
The corresponding nucleotide sequence was taken from:
K02205.1
Source
Zif268 and GCN4 [Wolfe00]| Sequence Annotation | Location | Component / Role(s) |
| Help:Barcodes Zif268: Finger 2 Zif268: Finger 3 (partial) linker GCN4: leucine zipper Finger2 Recognition Finger 3 Recognition | 268,292 7,90 91,150 151,165 166,261 43,63 127,147 | engineered_tag feature/barcode CDS feature/cds CDS feature/cds sequence_feature feature/misc CDS feature/cds feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |