BBa_I1022

BBa_I1022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I1022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman

Date created: 2003-01-31 12:00:00

Date modified: 2015-08-31 04:07:29

Antisense RNA w/BBa_I1020 Interference (micRNA)

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_I1022_sequence (Version 1)

Description

Sequence for antisense RNA that interferes with BBa_I1020 mRNA using the micRNA scheme.

Notes

References (unparsed) here:

Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970

Coleman, J., et al. Cell (1984) 37, 429-36

References (unparsed) here:

Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970

Coleman, J., et al. Cell (1984) 37, 429-36

BBa_I1022 antisense RNA is designed to interfere with BBa_I1020 mRNA via the micRNA scheme (Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970). The sequence is designed to interfere exclusively with BBa_I1020, and act independently of BBa_I1010 and BBa_I1012. These parts are designed to use codon space to code for the same protein (BBa_I1010 and BBa_1020 code for lambda cI) while allowing for independent anti-sense regulation of the BBa_I1010 and BBa_1020 transcripts via BBa_I1012 and BBa_1022.

Source

Anti-sense secondary structure adapted from: Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
stem_loop	1,57	stem_loop, feature/stem_loop
suffix	193,196	feature/misc, sequence_feature
stem_loop	158,192	feature/stem_loop, stem_loop
start	128,130	feature/start, start_codon
cI mRNA reverse complement	58,157	sequence_feature, feature/misc
cI CDS Reverse Complement	58,130	sequence_feature, feature/misc
RBS Reverse Complement	135,140	feature/misc, sequence_feature
BBa_I1022	1,196	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I1022/1

Member of these Collections

• //classic/rna/uncategorized
• iGEM Parts Registry