BBa_I1032

BBa_I1032 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I1032

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton

Date created: 2003-01-31 12:00:00

Date modified: 2015-08-31 04:07:29

CI(1) "
micRNA"
asRNA

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_I1032_sequence (Version 1)

Description

Region which serves as basis for transcription of asRNA that binds to and inhibits BBa_I1030's mRNA transcript. Has LacO-1 regulatory region and a Promoter. Part of the XOR gate comprised of BBa_I1030 and BBa_I1040, their corresponding asRNA coding sequences (BBa_I1012 and BBa_I1032).

Notes

Complementary to beginning of BBa_I1030 transcript covering junk region, RBS, start codon, and 73 bp into coding sequence. Two stem loops flank the antisense region. [KEIL96]

Incompatible with systems containing BBa_I1031
Compatible with BBa_I1020, BBa_I1021, BBa_I1022, BBa_I1023.

Source

see references

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
antisense region for I1030	113,220	sequence_feature, feature/misc
stem_loop	56,112	stem_loop, feature/stem_loop
stem_loop	221,255	stem_loop, feature/stem_loop
LacO-1	1,55	operator, feature/operator
BBa_I1032	1,255	engineered_region, feature/BioBrick

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I1032/1

Member of these Collections

• //classic/rna/uncategorized
• iGEM Parts Registry