Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: bu
Date created: 2004-09-07 11:00:00
Date modified: 2015-08-31 04:07:30
P21 half bit
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I11063_sequence (Version 1) |
Description
The P21 half bit uses integrase/excisionase from P21 and attP/attB (rev comp) from HK022. The attachment sites are flanking a unidirectional terminator. This half bit is designed to be used with the HK022 half bit (BBa_I11062)| Sequence Annotation | Location | Component / Role(s) |
| spacer SalI spacer RBS spacer Integrase p21 (BBa_I11050) LVA stop spacer SacII spacer HK022 attP attachment site (BBa_I11043) spacer MluI spacer BBa_I11013 terminator spacer ApaI spacer HK022 attB rev comp (BBa_I11042) spacer BamHI RBS spacer Excisionase p21 (BBa_I11051) AAV stop spacer XbaI spacer | 1,3 4,9 10,12 13,18 19,23 24,1163 1164,1196 1197,1202 1203,1205 1206,1211 1212,1214 1215,1443 1444,1446 1447,1452 1453,1455 1456,1584 1585,1587 1588,1593 1594,1596 1597,1647 1648,1650 1651,1656 1657,1662 1663,1667 1668,1901 1902,1934 1935,1940 1941,1943 1944,1949 1950,1951 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc ribosome_entry_site feature/rbs sequence_feature feature/misc CDS feature/cds feature/tag tag feature/stop stop_codon sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature stop_codon feature/stop sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc feature/rbs ribosome_entry_site feature/misc sequence_feature feature/cds CDS feature/tag tag feature/stop stop_codon feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc |