Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2006-03-13 12:00:00
Date modified: 2015-08-31 04:07:42
pSC101 origin of replication, reverse
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_I50041_sequence (Version 1) |
Description
pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040.| Sequence Annotation | Location | Component / Role(s) |
| putative polyA repA inverted repeat RBS RBS promoter region inverted repeat rep promoter -10 rep promoter -35 Mutated SpeI site direct repeat direct repeat direct repeat AT rich region par region inverted repeat inverted repeat inverted repeat Tn3 integration site inverted repeat terminator difference from canonical pSC101 sequence in Genbank difference from canonical pSC101 sequence in Genbank difference from canonical pSC101 sequence in Genbank difference from canonical pSC101 sequence in Genbank | 76,91 202,1152 1154,1176 1154,1159 1156,1168 1173,1192 1179,1200 1183,1188 1205,1210 1219,1224 1250,1269 1282,1301 1303,1322 1321,1403 1534,1901 1800,1817 1823,1846 1857,1873 1876,1880 1886,1899 1963,1992 36,36 126,126 288,288 383,383 | feature/polya polyA_site feature/cds CDS feature/misc sequence_feature feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs promoter feature/promoter sequence_feature feature/misc feature/promoter promoter promoter feature/promoter sequence_alteration feature/mutation feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/stem_loop stem_loop sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation feature/mutation sequence_alteration |